Generation of Controllable Gradients in Cell Density

• Type of Material: Article
• Authors: Wenying Liu, Georgia Institute of Technology; Yu Zhang, Georgia Institute of Technology; Starvos Thomopoulos, Washington University; Younan Xia, Emory University
• Language: English
• Date: 2013-01-01
• Publisher: Wiley: 12 months
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
• Final Published Version (URL): http://dx.doi.org/10.1002/anie.201206060
• Title of Journal or Parent Work: Angewandte Chemie International Edition
• ISSN: 1433-7851
• Volume: 52
• Issue: 1
• Start Page: 429
• End Page: 432
• Grant/Funding Information: This work was supported in part by a grant from the NIH (1R01 AR060820); and startup funds from Georgia Institute of Technology.
• Keywords: MIMICKING; FIBRONECTIN; FIBROBLASTS; BONE INSERTION SITE; cell adhesion; ATTACHMENT; Physical Sciences; interface tissue engineering; NANOFIBER SCAFFOLDS; ADHESION; Chemistry, Multidisciplinary; cell density gradient; SURFACES; Science & Technology; MIGRATION; PROTEINS; cell culture; Chemistry
• Research Categories: Chemistry, General; Engineering, Biomedical

Abstract

Making the grad(ient): A gradient in cell density was generated on a substrate that was inserted into a homogeneous suspension of cells at a specific tilt angle by taking advantage of the gradual change in the number of cells available for sedimentation (see scheme). Reverse gradients were also fabricated on the same substrate using multiple sedimentation procedures.