DNA repair pathway choice at various conditions immediately post irradiation

• Type of Material: Article
• Authors: Min Liu, Jilin University; Hongyan Wang, affiliation; Solah Lee, Emory University; Bailong Liu, Jilin University; Lihua Dong, Jilin University; Ya Wang, Emory University
• Language: English
• Date: 2016-01-01
• Publisher: Taylor & Francis
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2016 Informa UK Limited, trading as Taylor & Francis Group.
• Final Published Version (URL): http://dx.doi.org/10.1080/09553002.2016.1230243
• Title of Journal or Parent Work: International Journal of Radiation Biology
• ISSN: 0955-3002
• Volume: 92
• Issue: 12
• Start Page: 819
• End Page: 822
• Grant/Funding Information: This work is supported by grants from the National Aeronautics and Space Administration (NNX11AC30G to Y.W.) and the National Cancer Institute (CA186129, CA185882, to Y.W. and P30CA138292 to the Institute).
• Keywords: heavy ion; non-homologous end-joining (NHEJ); homologous recombination repair (HRR); HUMAN-TUMOR CELLS; ionizing radiation; Technology; Nuclear Science & Technology; Life Sciences & Biomedicine - Other Topics; MAMMALIAN-CELLS; HOMOLOGOUS RECOMBINATION; SUBLETHAL DAMAGE; sublethal damage repair (SLDR); DNA double strand break (DSB); Radiology, Nuclear Medicine & Medical Imaging; DEPENDENT PROTEIN-KINASE; Science & Technology; potentially lethal damage repair (PLDR); CONTRIBUTES; Biology; RADIATION; Life Sciences & Biomedicine; SENSITIVITY; STRAND BREAK REPAIR; DNA repair; POTENTIALLY LETHAL DAMAGE
• Research Categories: Biology, Genetics; Health Sciences, Radiology

Abstract

Purpose: To clarify which DNA double-strand break repair pathway, non-homologous end-joining (NHEJ), homologous recombination repair (HRR) or both, plays a key role in potentially lethal damage repair (PLDR). Methods and materials: Combining published data and our new potentially lethal damage repair (PLDR) data, we explain whether similar to sublethal damage repair (SLDR), PLDR also mainly depends on NHEJ versus HRR. The PLDR data used the same cell lines: wild type, HRR or NHEJ-deficient fibroblast cells, as those SLDR data published by our laboratory previously. The PLDR condition that we used was as commonly described by many other groups: the cells were collected immediately or overnight post ionizing radiation for colony formation after cultured to a plateau phase with a low concentration of serum medium. Results: Enough data from other groups and our lab showed that wild type or HRR-deficient cells had efficient PLDR, but NHEJ deficient cells did not. Conclusion: NHEJ contributes more to PLDR than HRR in mammalian cells, which is similar to SLDR. Since both SLDR and PLDR are relevant to clinical tumor status while undergoing radiotherapy, such clarification may benefit radiotherapy in the near future.