HIV Increases the Risk of Cigarette Smoke-Induced Emphysema Through MMP-9

• Type of Material: Article
• Authors: Bashar Staitieh, Emory University; Simran Malik, Washington University in St. Louis; Sara Auld, Emory University; Gregory W. Wigger, Emory University; Xian Fan, Emory University; Andrew T. Roth, Washington University; Tanima Chatterjee, University of Alabama Birmingham; Itika Arora, University of Alabama Birmingham; S. Vamsee Raju, University of Alabama Birmingham; Sonya Heath, University of Alabama Birmingham; Saurabh Aggrawal, University of Alabama Birmingham
• Language: English
• Date: 2023-03-01
• Publisher: LIPPINCOTT WILLIAMS & WILKINS
• Publication Version: Final Published Version
• Copyright Statement: © 2022 The Author(s). Published by Wolters Kluwer Health, Inc.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1097/QAI.0000000000003125
• Title of Journal or Parent Work: JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
• Volume: 92
• Issue: 3
• Start Page: 263
• End Page: 270
• Grant/Funding Information: This work was supported by grants from the National Institutes of Health: K08 AA 024512 (B.S.S.), K23 AI134182 (S.C.A.), T32 HL116271 (G.W.W.), 1R01DA049657, and U01 ES033265 (S.A.).
• Keywords: Life Sciences & Biomedicine; ERA; WOMEN; DEATH; INFECTION; RAT; MATRIX METALLOPROTEINASES; emphysema; Tat; metalloproteinase; smoking; Science & Technology; Immunology; Infectious Diseases; HIV
• Research Categories: Health Sciences, Public Health; Health Sciences, Immunology

Abstract

Background:HIV is associated with an increased risk for emphysema. Matrix metalloproteinase 9 (MMP-9) is a lung tissue remodeling enzyme associated with emphysema. We previously found MMP-9 activity increases with increases in oxidative stress and that HIV increases alveolar oxidative stress. We hypothesized that HIV proteins would increase the risk of cigarette smoke-induced emphysema due to MMP-9.Methods:HIV-1 transgenic rats and wild-type littermates were exposed to cigarette smoke or sham for 8 weeks. Lung compliance and histology were assessed. Bronchoalveolar lavage (BAL), primary alveolar macrophages (AM), and serum samples were obtained. A rat alveolar macrophage cell line was exposed to the HIV protein Tat, and MMP-9 levels were assessed by Western immunoblotting. MMP-9 protein expression and activity were assessed in AM from the HIV rat model by ELISA and cytoimmunofluoresence, respectively. Serum from human subjects with and without HIV and tobacco dependence was assessed for MMP-9 levels.Results:MMP-9 expression was significantly increased in rat alveolar macrophages after Tat exposure. HIV-1 transgenic rats developed emphysema while wild-type littermates did not. MMP-9 expression was also increased in the serum, BAL, and AM of HIV-1 transgenic rats after exposure to cigarette smoke compared with wild-type rats. In parallel, serum samples from HIV+ smokers had higher levels of MMP-9 than subjects without HIV and those who did not smoke.Conclusion:The combination of HIV and cigarette smoke increases MMP-9 expression in experimental rat HIV models and human subjects. HIV and cigarette smoke both induce alveolar oxidative stress and thereby increase MMP-9 activity.