Cross-validation of biomonitoring methods for polycyclic aromatic hydrocarbon metabolites in human urine: Results from the formative phase of the Household Air Pollution Intervention Network (HAPIN) trial in India

• Type of Material: Article
• Authors: Naveen Puttaswamy, Sri Ramachandra Institute of Higher Education & Research; Sudhakar Saidam, Sri Ramachandra Institute of Higher Education & Research; Gayathri Rajendran, Sri Ramachandra Institute of Higher Education & Research; Kokila Arumugam, Sri Ramachandra Institute of Higher Education & Research; Savannah Gupton, Emory University; Erin W. Williams, Emory University; Cierra L. Johnson, Emory University; Parinya Panuwet, Emory University; Sarah Rajkumar, Colorado State University; Maggie L. Clark, Colorado State University; Jennifer L. Peel, Colorado State University; William Checkley, Johns Hopkins University; Thomas Clasen, Emory University; Kalpana Balakrishnan, Sri Ramachandra Institute of Higher Education & Research; Dana Barr, Emory University
• Language: English
• Date: 2020-10-01
• Publisher: ELSEVIER
• Publication Version: Final Published Version
• Copyright Statement: © 2020 The Authors
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.jchromb.2020.122284
• Title of Journal or Parent Work: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
• Volume: 1154
• Start Page: 122284
• End Page: 122284
• Grant/Funding Information: The HAPIN trial is funded by the U.S. National Institutes of Health (cooperative agreement 1UM1HL134590) in collaboration with the Bill & Melinda Gates Foundation [OPP1131279]. Additional support was provided by Emory’s NIH-funded Human Exposome Research Center (P30ES019776).
• Keywords: BIOMARKERS; BIRTH OUTCOMES; 2-naphthol,1-hydroxypyrene; Cross-validation; Chemistry; Biochemical Research Methods; PAHS; EMISSIONS; Physical Sciences; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; ASSOCIATION; PRENATAL EXPOSURE; Chemistry, Analytical; HPLC-fluorescence; PREGNANCY; Polycyclic aromatic hydrocarbons; CHILD IQ; 1-HYDROXYPYRENE; Science & Technology; PERFORMANCE LIQUID-CHROMATOGRAPHY; Household air pollution
• Research Categories: Biology, Molecular; Chemistry, Analytical; Chemistry, Biochemistry

Abstract

The Household Air Pollution Intervention Network (HAPIN) trial is evaluating health benefits of a liquefied petroleum gas (LPG) stove intervention in biomass cook-fuel using homes (n = 3200) in four low-and middle-income countries (LMICs) that include Peru, Guatemala, Rwanda and India. Longitudinal urine samples (n = 6000) collected from enrolled pregnant women, infants and older women will be analyzed for biomarkers associated with exposure and health outcomes. We report results from cross-validation of a lower cost high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method with a higher resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the measurement of 1-hydroxypyrene (1PYR) and 2-naphthol (2NAP). Twenty-five split urine samples were analyzed by HPLC-FLD method at the India trial site in Chennai, India and by LC-MSMS method at the trial wide Biomarker Coordinating Center, Emory University, USA. The limits of detection (LOD) for the HPLC-FLD method were 0.02 ng/mL and 0.07 ng/mL for 2NAP and 1PYR, respectively. Bland-Altman analysis estimated a bias of 2.98 ng/ml for 2NAP (95% CI: −5.22, −0.75) and 0.09 ng/mL for 1PYR (95% CI: −0.02, 0.21) with HPLC-FLD levels being lower than LC-MSMS levels at higher concentrations. Analyses of additional urine samples (n = 119) collected during the formative phase of the HAPIN trial in India, showed 2NAP and 1PYR levels to be consistently above the limit of quantification (LOQ) and demonstrated the applicability of the method. The HPLC-FLD method can serve as a cost-effective and reliable analytical method to measure 2NAP and 1PYR in human urine in LMICs, within and beyond the HAPIN trial.