Zika virus directly infects peripheral neurons and induces cell death

• Type of Material: Article
• Authors: Yohan Oh, Johns Hopkins University; Feiran Zhang, Emory University; Yaqing Wang, Chinese Academy of Sciences; Emily M. Lee, Florida State University; In Young Choi, Johns Hopkins University; Hotae Lim, Johns Hopkins University; Fahimeh Mirakhori, Johns Hopkins University; Ronghua Li, Emory University; Luoxiu Huang, Emory University; Tianlei Xu, Emory University; Hao Wu, Emory University; Cui Li, affiliation; Cheng-Feng Qin, Beijing Institute of Microbiology and Epidemiology; Zhexing Wen, Johns Hopkins University; Qing-Feng Wu, Chinese Academy of Sciences; Hengli Tang, Florida State University; Zhiheng Xu, Chinese Academy of Sciences; Peng Jin, Emory University; Hongjun Song, Johns Hopkins University; Guo-li Ming, Johns Hopkins University; Gabsang Lee, Johns Hopkins University
• Language: English
• Date: 2017-09-01
• Publisher: Nature Publishing Group
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
• Final Published Version (URL): http://dx.doi.org/10.1038/nn.4612
• Title of Journal or Parent Work: Nature Neuroscience
• ISSN: 1097-6256
• Volume: 20
• Issue: 9
• Start Page: 1209
• End Page: +
• Grant/Funding Information: This work was supported by grants from the Robertson Investigator Award from New York Stem Cell Foundation (G.L.), Maryland Stem Cell Research Funding (MSCRF; G.L., H.S., G.M.), NIH R01NS093213 (G.L.), R01MH105128 and R35NS097370 (G.M.), U19AI131130 (G.M., Z.W., H.T., and P.J.), U19MH106434, P01NS097206, R37NS047344 (H.S.), R21AI119530 (H.T.), NSF (China) (31430037), MOST (China) “973” program (2014CB942801/2012YQ03026006; Y.W., Z.X.), FSU Zika seed funding (H.T.), and by Adelson Medical Research Foundation (G.M.).; The authors (G.L., Y.O., G.M., H.S.) acknowledge the joint participation by the Adrienne Helis Malvin Medical Research Foundation.
• Keywords: PROGENITORS; EMBRYONIC STEM-CELLS; EXPOSURE; BRAIN; IDENTIFICATION; Science & Technology; NEURAL CREST CELLS; MODELS; Life Sciences & Biomedicine; Neurosciences; DERIVATION; Neurosciences & Neurology
• Research Categories: Health Sciences, Public Health; Biology, Virology

Abstract

Zika virus (ZIKV) infection is associated with neurological disorders of both the CNS and peripheral nervous systems (PNS), yet few studies have directly examined PNS infection. Here we show that intraperitoneally or intraventricularly injected ZIKV in the mouse can infect and impact peripheral neurons in vivo. Moreover, ZIKV productively infects stem-cell-derived human neural crest cells and peripheral neurons in vitro, leading to increased cell death, transcriptional dysregulation and cell-type-specific molecular pathology.