Cell Synchronization by Double Thymidine Block.

• Type of Material: Article
• Authors: Guo Chen, Emory University; Xingming Deng, Emory University
• Language: English
• Date: 2018-09-05
• Publisher: bio-protocol
• Publication Version: Final Published Version
• Copyright Statement: © 2018 The Authors; exclusive licensee Bio-protocol LLC.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.21769/BioProtoc.2994
• Title of Journal or Parent Work: Bio Protoc
• Volume: 8
• Issue: 17
• Grant/Funding Information: This work was supported by NIH/NCI grants R01CA193828, R01CA136534 and R01CA200905 (to X. Deng), by the Winship Research Pathology and Integrated Cellular Imaging shared resource and the Emory Comprehensive Glycomics Core (ECGC) supported by the Winship Cancer Institute of Emory University (P30CAJ 38292), by the Winship Fashion a Cure Research Scholar Award (to X. Deng), a philanthropic award provided by the Winship, and by the Winship Endowment Fund (to XD).
• Keywords: Cell synchronization; DNA synthesis; Thymidine; Cell cycle; DNA replication
• Research Categories: Health Sciences, Oncology

Abstract

Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.