Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue

• Type of Material: Article
• Authors: Prestina Smith, Emory University; Lauren A Jeffers, Emory University; Michael Koval, Emory University
• Language: English
• Date: 2021-01-01
• Publisher: HUMANA PRESS INC
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2021 Springer Science+Business Media, LLC
• Final Published Version (URL): http://dx.doi.org/10.1007/7651_2020_345
• Title of Journal or Parent Work: PERMEABILITY BARRIER, 2 EDITION
• Volume: 2367
• Start Page: 137
• End Page: 148
• Grant/Funding Information: Supported by a Ford Foundation Fellowship (PS), NIH grants R01-AA025854 and R01-HL137112 (MK), and F31-HL149323 (LAJ).
• Keywords: Biochemical Research Methods; Vascular endothelium; Science & Technology; Biochemistry & Molecular Biology; RECEPTOR; Pulmonary edema; Cell Biology; Alveolar epithelium; Life Sciences & Biomedicine; Physiology; Tight junctions; Lung barrier function; ALBUMIN; Acute respiratory distress syndrome

Abstract

Lung fluid balance is maintained in part by the barriers formed by the pulmonary microvasculature and alveolar epithelium. Failure of either of these barriers leads to pulmonary edema, which limits lung function and exacerbates the severity of acute lung injury. Here we describe a method using Evans Blue dye to simultaneously measure the function of vascular and epithelial barriers of murine lungs in vivo.