Mutations within the rplD Gene of Linezolid-Nonsusceptible Streptococcus pneumoniae Strains Isolated in the United States

• Type of Material: Article
• Authors: W Dong, Emory University; S Chochua, Emory University; L McGee, Emory University; D Jackson, Emory University; Keith Klugman, Emory University; Jorge Vidal Graniel, Emory University
• Language: English
• Date: 2014-04-01
• Publisher: American Society for Microbiology
• Publication Version: Final Published Version
• Copyright Statement: © 2014, American Society for Microbiology. All Rights Reserved.
• Final Published Version (URL): http://dx.doi.org/10.1128/AAC.02630-13
• Title of Journal or Parent Work: Antimicrobial Agents and Chemotherapy
• ISSN: 0066-4804
• Volume: 58
• Issue: 4
• Start Page: 2459
• End Page: 2462
• Keywords: LEADER PROGRAM; RIBOSOMAL-PROTEIN L4; MECHANISM; Life Sciences & Biomedicine; CHLORAMPHENICOL; IN-VITRO; RESISTANT STAPHYLOCOCCUS-AUREUS; SPECTRUM; Pharmacology & Pharmacy; Science & Technology; CFR; 1ST REPORT; Microbiology; SURVEILLANCE PROGRAM
• Research Categories: Health Sciences, Epidemiology; Health Sciences, Public Health

Abstract

Three invasive Streptococcus pneumoniae strains nonsusceptible to linezolid were isolated in the United States between 2001 and 2012 from the CDC's Active Bacterial Core surveillance. Linezolid binds ribosomal proteins where structural changes within its target site may confer resistance. Our study identified mutations and deletions near the linezolid binding pocket of two of these strains within the rplD gene, which encodes ribosomal protein L4. Mutations in the 23S rRNA alleles or the rplV gene were not detected.