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Surveillance of Diarrheagenic Escherichia coli Strains Isolated from Diarrhea Cases from Children, Adults and Elderly at Northwest of Mexico

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Last modified
  • 02/20/2025
Type of Material
Authors
    Adrian Canizalez-Roman, Autonomous University of SinaloaHéctor M. Flores-Villaseñor, Autonomous University of SinaloaEdgar Gonzalez-Nuñez, Secretariat of Health, MexicoJorge Velazquez-Roman, Autonomous University of SinaloaJorge Vidal Graniel, Emory UniversitySecundino Muro-Amador, Autonomous University of SinaloaGerardo Alapizco-Castro, Autonomous University of SinaloaJ. Alberto Díaz-Quiñonez, Instituto de Diagnóstico y Referencia EpidemiológicosNidia León-Sicairos, Autonomous University of Sinaloa
Language
  • English
Date
  • 2016-11-30
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2016 Canizalez-Roman, Flores-Villaseñor, Gonzalez-Nuñez, Velazquez-Roman, Vidal, Muro-Amador, Alapizco-Castro, Díaz-Quiñonez and León-Sicairos.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1664-302X
Volume
  • 7
Start Page
  • 1924
End Page
  • 1924
Grant/Funding Information
  • This work was supported by grants from PROFAPI 2014/038 (Autonomous University of Sinaloa) to AC-R and PROMEP/2014 DSA/103.5/14/11063 (SEP), FOLIO UAS NPTC-11 to HF-V.
Supplemental Material (URL)
Abstract
  • Diarrheagenic Escherichia coli (DEC) strains are a main cause of gastrointestinal disease in developing countries. In this study we report the epidemiologic surveillance in a 4-year period (January 2011 to December 2014) of DEC strains causing acute diarrhea throughout the Sinaloa State, Mexico. DEC strains were isolated from outpatients of all ages with acute diarrhea (N = 1,037). Specific DEC pathotypes were identified by PCR-amplification of genes encoding virulence factors. The adhesion phenotype and antibiotic resistance were also investigated. DEC strains were detected in 23.3% (242/1037) of cases. The most frequently DEC strain isolated was EAEC [(12.2%), 126/242] followed by EPEC [(5.1%), 53/242], ETEC [(4.3%), 43/242] DAEC [(1.4%), 15/242], STEC [(0.3%), 3/242], and EIEC [(0.2%), 2/242]. EHEC strains were not detected. Overall DEC strains were more prevalent in children ≤2 years of age with EPEC strains the most common of DEC pathotypes. While ∼65% of EAEC strains were classified as typical variant based on the aggregative adherence to in vitro cultures of HEp-2 cells, a high proportion of EPEC strains was classified as atypical strains. EAEC, EPEC, ETEC, and DAEC strains were distributed in the north, central and south regions of Sinaloa state. Among all DEC strains, >90% were resistant to at least one commonly prescribed antibiotic. Strains were commonly resistant to first-line antibiotics such as tetracycline, ampicillin, and sulfamethoxazole-trimethoprim. Furthermore, more than 80% of DEC isolates were multi-drug resistant and EPEC and DAEC were the categories with major proportion of this feature. In conclusion, in nearly one out of four cases of acute diarrhea in Northwestern Mexico a multi-drug resistant DEC strain was isolated, in these cases EAEC was the most prevalent (52%) pathotype.
Author Notes
Keywords
Research Categories
  • Biology, Microbiology
  • Health Sciences, Epidemiology

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