Ultraviolet Hyperspectral Interferometric Microscopy

• Type of Material: Article
• Authors: Ashkan Ojaghi, Georgia Institute of Technology; Meredith E. Fay, Georgia Institute of Technology; Wilbur Lam, Emory University; Francisco E. Robles, Emory University
• Language: English
• Date: 2018-07-02
• Publisher: Nature Research (part of Springer Nature): Fully open access journals
• Publication Version: Final Published Version
• Copyright Statement: © 2018 The Author(s).
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41598-018-28208-0
• Title of Journal or Parent Work: Scientific Reports
• ISSN: 2045-2322
• Volume: 8
• Issue: 1
• Start Page: 9913
• End Page: 9913
• Grant/Funding Information: We greatly acknowledge support for this work by the National Science Foundation (NSF CBET CAREER 1752011), Massner Lane Family, Burroughs Wellcome Fund (CASI BWF1014540), and Georgia Institute of Technology.
• Keywords: SPECTROSCOPY; OBJECTS; Science & Technology; Science & Technology - Other Topics; Multidisciplinary Sciences; REFRACTIVE-INDEX; PHASE; INTRACELLULAR PROTEIN; REFRACTOMETRY; NUCLEIC-ACID
• Research Categories: Health Sciences, Medicine and Surgery; Engineering, Biomedical

Abstract

Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells.