Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species▿ †

• Type of Material: Article
• Authors: Shana Topp, Emory University; Colleen M. K. Reynoso, Emory University; Jessica C. Seeliger, University of California, Berkeley; Ian S. Goldlust, Emory University; Shawn K. Desai, Emory University; Dorothee Murat, University of California, Berkeley; Aimee Shen, Stanford University; Aaron W. Puri, Stanford University; Arash Komeili, University of California, Berkeley; Carolyn R. Bertozzi, University of California, Berkeley; June R Scott, Emory University; Justin Gallivan, Emory University
• Language: English
• Date: 2010-12
• Publisher: American Society for Microbiology (ASM)
• Publication Version: Final Published Version
• Copyright Statement: © 2010, American Society for Microbiology
• Final Published Version (URL): http://aem.asm.org/content/76/23/7881
• Title of Journal or Parent Work: Applied and Environmental Microbiology
• Volume: 76
• Issue: 23
• Start Page: 7881
• End Page: 7884
• Grant/Funding Information: This work was supported in part by NIH grants (GM074070 to J.P.G., AI20723 to J.R.S., GM084122 to A.K., and AI51622 to C.R.B.).
• Research Categories: Chemistry, General; Biology, Microbiology; Biology, Cell

Abstract

We developed a series of ligand-inducible riboswitches that control gene expression in diverse species of Gram-negative and Gram-positive bacteria, including human pathogens that have few or no previously reported inducible expression systems. We anticipate that these riboswitches will be useful tools for genetic studies in a wide range of bacteria.