Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer

• Type of Material: Article
• Authors: Lihan Zhao, Georgia State University; Manali Rupji, Emory University; Ishita Choudhary, Georgia State University; Remus Osan, Georgia State University; Shobhna Kapoor, Indian Institute of Technology; Hong-Jie Zhang, Hong Kong Baptist University; Chunhua Yang, Georgia State University; Ritu Aneja, Georgia State University
• Language: English
• Date: 2020-11-05
• Publisher: NATURE RESEARCH
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2020
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41598-020-75707-0
• Title of Journal or Parent Work: SCIENTIFIC REPORTS
• Volume: 10
• Issue: 1
• Start Page: 19182
• End Page: 19182
• Grant/Funding Information: This research was funded by National Institutes of Health, Grant Number R01 CA169127 to RA. Research reported in this publication was supported in part by the Biostatistics and Bioinformatics Shared Resource of Winship Cancer Institute of Emory University and NIH/NCI under award number P30CA138292. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
• Keywords: PHYTOCHEMICALS; Multidisciplinary Sciences; NORMALIZATION; CELLS; Science & Technology; CHALLENGES; CHEMOMETRICS; Science & Technology - Other Topics
• Research Categories: Chemistry, Biochemistry; Biology, Cell

Abstract

Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC50) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC50 values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts.