Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms

• Type of Material: Article
• Authors: Colleen M. McDowell, University of Wisconsin; Krishnakumar Kizhatil, Jackson Laboratory; Michael H. Elliott, University of Oklahoma; Darryl R. Overby, Imperial College London; Joseph Van Batenburg-Sherwood, Imperial College London; J. Cameron Millar, University of North Texas; Markus H. Kuehn, University of Iowa; Michael P. Fautsch, Mayo Clinic; ER Tamm, University of Regensburg; Christopher Ethier, Emory University; W. Daniel Stamer, Duke University
• Language: English
• Date: 2022-02-01
• Publisher: ARVO Publishing
• Publication Version: Final Published Version
• Copyright Statement: © 2022 The Authors.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1167/iovs.63.2.12
• Title of Journal or Parent Work: Investigative Ophthalmology & Visual Science
• Volume: 63
• Issue: 2
• Start Page: 12
• End Page: 12
• Grant/Funding Information: The authors thank Aerie Pharmaceuticals, Inc.; Aerpio Pharmaceuticals, Inc.; Allergan; Bausch + Lomb; BrightFocus Foundation; Glaucoma Research Foundation; Glaukonix, Inc.;; Glaukos Corp.; Nicox Visible Science; Novartis Pharmaceuticals, Inc.; Regeneron Pharmaceuticals; Santen, Inc.; and the Western Glaucoma Foundation for continued support of the trabecular meshwork study club, enabling 20 annual scientific gatherings of researchers dedicated to better understanding trabecular meshwork biology and facilitating the assembly of this position paper; The authors also thank BrightFocus Foundation for supporting the virtual trabecular meshwork study club meeting to enable continuous discussion among researchers throughout the COVID-19 pandemic.
• Keywords: Human eyes; mouse model; conventional outflow; ocular hypertension; intraocular pressure; Life Sciences & Biomedicine; Extracellular matrix; Open angle glaucoma; Trabecular meshwork cells; Hydrogen sulfide; Intraocular pressure; Science & Technology; glaucoma; Noninvasive stimulation; Optic nerve damage; outflow facility; Ophthalmology; Sympathetic neurotransmission; Rescues glaucoma
• Research Categories: Biology, Neuroscience; Health Sciences, Opthamology; Biology, Cell; Engineering, Biomedical

Abstract

Due to their similarities in anatomy, physiology, and pharmacology to humans, mice are a valuable model system to study the generation and mechanisms modulating conventional outflow resistance and thus intraocular pressure. In addition, mouse models are critical for understanding the complex nature of conventional outflow homeostasis and dysfunction that results in ocular hypertension. In this review, we describe a set of minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. We expect that this set of standard practices will increase scientific rigor when using mouse models and will better enable researchers to replicate and build upon previous findings.