Systems Analysis of Immunity to Influenza Vaccination across Multiple Years and in Diverse Populations Reveals Shared Molecular Signatures

Helder, Nakaya, Emory University; Thomas, Hagan, University of California San Diego; Sai S., Duraisingham, Oxford University; Eva K., Lee, Georgia Institute of Technology; Marcin, Kwissa, University of Chicago; Nadine, Rouphael, Emory University; Daniela, Frasca, University of Miami; Merril, Gersten, University of California San Diego; Aneesh, Mehta, Emory University; Renaud, Gaujoux, Technion-Israel Institute of Technology; Gui-Mei, Li, Emory University; Shakti, Gupta, University of California San Diego; Rafi, Ahmed, Emory University; Mark, Mulligan, Emory University; Shai, Shen-Orr, Technion-Israel Institute of Technology; Bonnie B., Blomberg, University of Miami; Shankar, Subramaniam, University of California San Diego; Bali, Pulendran, Emory University

Persistent URL

https://open.library.emory.edu/purl/2902z34tt4-emory

Last modified

02/20/2025

Type of Material

Article

Authors

Helder Nakaya, Emory University

Thomas Hagan, University of California San Diego

Sai S. Duraisingham, Oxford University

Eva K. Lee, Georgia Institute of Technology

Marcin Kwissa, University of Chicago

Nadine Rouphael, Emory UniversityDaniela Frasca, University of MiamiMerril Gersten, University of California San DiegoAneesh Mehta, Emory UniversityRenaud Gaujoux, Technion-Israel Institute of TechnologyGui-Mei Li, Emory UniversityShakti Gupta, University of California San DiegoRafi Ahmed, Emory UniversityMark Mulligan, Emory UniversityShai Shen-Orr, Technion-Israel Institute of TechnologyBonnie B. Blomberg, University of MiamiShankar Subramaniam, University of California San DiegoBali Pulendran, Emory University

Language

English

Date

2015-12-15

Publisher

Elsevier (Cell Press)

Publication Version

Author Accepted Manuscript (After Peer Review)

Copyright Statement

© 2015 Elsevier Inc.

Final Published Version (URL)

http://dx.doi.org/10.1016/j.immuni.2015.11.012

Title of Journal or Parent Work

Immunity

ISSN

1074-7613

Volume

43

Issue

6

Start Page

1186

End Page

1198

Grant/Funding Information

H.I.N. receives a CNPq (Brazilian Research Council) research fellowship.
This work was supported by NIH grants U19AI090023 (B.P.), U19AI057266 (R.A. and B.P.), HHSN272201400004C (B.P.), U54AI057157 (B.P.), R37AI48638 (B.P.), and R37DK057665 (B.P.) and NSF Grant STC-0939370 (S.S.).

Supplemental Material (URL)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859820/bin/NIHMS782906-supplement-supplementary_figs.pdf

Abstract

Systems approaches have been used to describe molecular signatures driving immunity to influenza vaccination in humans. Whether such signatures are similar across multiple seasons and in diverse populations is unknown. We applied systems approaches to study immune responses in young, elderly, and diabetic subjects vaccinated with the seasonal influenza vaccine across five consecutive seasons. Signatures of innate immunity and plasmablasts correlated with and predicted influenza antibody titers at 1 month after vaccination with >80% accuracy across multiple seasons but were not associated with the longevity of the response. Baseline signatures of lymphocyte and monocyte inflammation were positively and negatively correlated, respectively, with antibody responses at 1 month. Finally, integrative analysis of microRNAs and transcriptomic profiling revealed potential regulators of vaccine immunity. These results identify shared vaccine-induced signatures across multiple seasons and in diverse populations and might help guide the development of next-generation vaccines that provide persistent immunity against influenza.

Author Notes