Development of a Specimen-Sparing Multichannel Bead Assay to Detect Antiparasite IgG4 for the Diagnosis of Schistosoma and Wuchereria Infections on the Coast of Kenya

• Type of Material: Article
• Authors: Adam S. DuVall, University of Michigan; Jessica Fairley, Emory University; Laura Sutherland, Case Western Reserve University; Amaya L. Bustinduy, Liverpool School of Tropical Medicine; Peter L Mungai, Case Western Reserve University; Eric M Muchiri, Division of Vector Borne and Neglected Tropical Diseases; Indu Malhotra, Case Western Reserve University; Uriel Kitron, Emory University; Charles H. King, Case Western Reserve University
• Language: English
• Date: 2014-04-01
• Publisher: American Society of Tropical Medicine and Hygiene
• Publication Version: Final Published Version
• Copyright Statement: ©The American Society of Tropical Medicine and Hygiene
• Final Published Version (URL): http://dx.doi.org/10.4269/ajtmh.13-0292
• Title of Journal or Parent Work: American Journal of Tropical Medicine and Hygiene
• ISSN: 0002-9637
• Volume: 90
• Issue: 4
• Start Page: 638
• End Page: 645
• Grant/Funding Information: This study was supported by National Institutes of Health Research Grant R01TW008067, which was funded by the Ecology of Infectious Diseases Program of the Fogarty International Center.
• Research Categories: Biology, General; Health Sciences, Public Health

Abstract

To better delineate the impact of parasitic coinfection in coastal Kenya, we developed a novel specimen-sparing bead assay using multiplex flow immunoassay (MFI) technology to simultaneously measure serum or plasma immunoglobulin G4 (IgG4) against Brugia malayi antigen (BMA) and Schistosoma haematobium soluble worm antigen (SWAP). Properties of the bead assay were estimated by latent class analysis using data from S. haematobium egg counts/filarial rapid diagnostic cards (RDTs), parasite-specific enzyme-linked immunosorbent assays (ELISAs), and the multichannel IgG4 assay. For schistosomiasis, the bead assay had an estimated sensitivity of 81% and a specificity of 45%, and it was more sensitive than ELISA or urine egg counts for diagnosing infection. For filariasis, it had a sensitivity of 86% and a specificity of 39%, and it was more sensitive than ELISA or RDT. Measuring antibody by MFI is feasible and may provide more accurate epidemiological information than current parasitological tests, especially in the setting of low-intensity infections. Copyright © 2014 by The American Society of Tropical Medicine and Hygiene.