Analysis of Mouse RPE Sheet Morphology Gives Discriminatory Categories

• Type of Material: Article
• Authors: Yi Jiang, Georgia State University; X Qi, Georgia State University; Micah A. Chrenek, Emory University; Christopher Gardner, Emory University; Nupur Dalal, Emory University; Jeffrey Boatright, Emory University; Hans Grossniklaus, Emory University; John Nickerson, Emory University
• Language: English
• Date: 2014
• Publisher: Springer Verlag (Germany)
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2014, Springer Science+Business Media, LLC
• Final Published Version (URL): http://link.springer.com/chapter/10.1007%2F978-1-4614-3209-8_76
• Title of Journal or Parent Work: Advances in Experimental Medicine and Biology
• ISSN: 0065-2598
• Volume: 801
• Start Page: 601
• End Page: 607
• Grant/Funding Information: Supported by NIH grants R01EY0016470, and P30EY007092, and an unrestricted grant to the Emory Eye Center from Research to Prevent Blindness.
• Keywords: pattern analysis; Retinal pigmented epithelium; morphometrics; RPE
• Research Categories: Health Sciences, Opthamology; Health Sciences, General

Abstract

We are interested in developing quantitative tools to study RPE morphology. We want to detect changes in the RPE by strain, disease, genotype, and age. Ultimately these tools should be useful in predicting retinal disease progression. The morphometric data will also help us to understand RPE sheet formation and barrier functions. A clear disruption of the regular cell size and shape appeared in mouse mutants. Aspect ratio and cell area together gave rise to principal components that predicted age and genotype accurately and well before visually obvious damage could be seen.