Aging-regulated TUG1 is dispensable for endothelial cell function

• Type of Material: Article
• Authors: Anna Theresa Gimbel, Goethe-University; Susanne Koziarek, Goethe-University; Kosta Theodorou, Goethe-University; Jana Felicitas Schulz, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC); Laura Stanicek, Goethe-University; Veerle Kremer, Vrije Universiteit Amsterdam; Tamer Ali, Goethe-University; Stefan Günther, DZHK (German Centre for Cardiovascular Research); Sandeep Kumar, Emory University; Hanjoong Jo, Emory University; Norbert Hübner, Max Delbruck Ctr Mol Med Helmholtz Assoc MDC; Lars Maegdefessel, Technical University of Munich; Stefanie Dimmeler, Goethe-University; Sebastiaan van Heesch, Princess Máxima Center for Pediatric Oncology; Reinier A Boon, Goethe-University
• Language: English
• Date: 2022-09-29
• Publisher: PUBLIC LIBRARY SCIENCE
• Publication Version: Final Published Version
• Copyright Statement: © 2022 Gimbel et al
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0265160
• Title of Journal or Parent Work: PLOS ONE
• Volume: 17
• Issue: 9
• Start Page: e0265160
• End Page: e0265160
• Grant/Funding Information: RB was supported by the Rembrandt Institute of Cardiovascular Science, the German Centre for Cardiovascular Research (DZHK), the European Research Council (ERC starting grant “NOVA” and consolidator grant “NICCA”), the European Union (Horizon 2020 Grant No. 825670), the Cardiopulmonary Institute (CPI) and the SFB834 and TRR267 of the German Research Council (DFG). NH was supported by the ERC Advanced Grant “CodingHeart”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• Keywords: Science & Technology - Other Topics; NONCODING RNA TUG1; Multidisciplinary Sciences; ANGIOGENESIS; CLASSIFICATION; Science & Technology; GENE
• Research Categories: Engineering, Biomedical

Abstract

The evolutionary conserved Taurine Upregulated Gene 1 (TUG1) is a ubiquitously expressed gene that is one of the highest expressed genes in human and rodent endothelial cells (ECs). We here show that TUG1 expression decreases significantly in aging mouse carotid artery ECs and human ECs in vitro, indicating a potential role in the aging endothelial vasculature system. We therefore investigated if, and how, TUG1 might function in aging ECs, but despite extensive phenotyping found no alterations in basal EC proliferation, apoptosis, barrier function, migration, mitochondrial function, or monocyte adhesion upon TUG1 silencing in vitro. TUG1 knockdown did slightly and significantly decrease cumulative sprout length upon vascular endothelial growth factor A stimulation in human umbilical vein endothelial cells (HUVECs), though TUG1-silenced HUVECs displayed no transcriptomewide mRNA expression changes explaining this effect. Further, ectopic expression of the highly conserved and recently discovered 153 amino acid protein translated from certain TUG1 transcript isoforms did not alter angiogenic sprouting in vitro. Our data show that, despite a high expression and strong evolutionary conservation of both the TUG1 locus and the protein sequence it encodes, TUG1 does not seem to play a major role in basic endothelial cell function.