Overproduction of the MtrCDE Efflux Pump in Neisseria gonorrhoeae Produces Unexpected Changes in Cellular Transcription Patterns

• Type of Material: Article
• Authors: Elizabeth A. Ohneck, Emory University; Maira Goytia, Emory University; Corinne Loughlin, Emory University; Sandeep J. Joseph, Emory University; Timothy Read, Emory University; Ann E. Jerse, Uniformed Services University of the Health Sciences; William Shafer, Emory University
• Language: English
• Date: 2015-01-01
• Publisher: American Society for Microbiology
• Publication Version: Final Published Version
• Copyright Statement: © 2015, American Society for Microbiology
• License: Creative Commons Attribution-Noncommercial 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1128/AAC.04148-14
• Title of Journal or Parent Work: Antimicrobial Agents and Chemotherapy
• ISSN: 0066-4804
• Volume: 59
• Issue: 1
• Start Page: 724
• End Page: 726
• Grant/Funding Information: This work was supported by NIH grants AI021150-29 (W.M.S.), AI42053 (A.E.J.), AI096788 (T.D.R., D. Dean, and R. Selden), and AI031496-22 (to P. F. Sparling, University of North Carolina) and a VA Merit Review Grant to W.M.S.
• Research Categories: Biology, Microbiology; Health Sciences, General

Abstract

The global consequence of drug efflux gene overexpression in bacteria has not been specifically analyzed because strains showing high-level expression typically have mutations in genes encoding regulatory proteins that control other genes. Results from a transcriptional profiling study performed with a strain of Neisseria gonorrhoeae that is capable of high-level transcription of the mtrCDE efflux pump operon independently of control by cognate regulatory proteins revealed that its overexpression has ramifications for systems other than drug efflux.