The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data

• Type of Material: Article
• Authors: Yan Liu, Imperial College London; Ryan McBride, Scripps Research Institute; Mark Stoll, Imperial College London; Angelina S Palma, Imperial College London; Lisete Silva, Imperial College London; Sanjay Agravat, Harvard Medical School; Kiyoko F Aoki-Kinoshita, Soka University; Matthew P Campbell, Macquarie University; Catherine E Costello, Boston University; Anne Dell, Imperial College London; Stuart M Haslam, Imperial College London; Niclas G Karlsson, University of Gothenburg; Kay-Hooi Khoo, Acad Sinica; Daniel Kolarich, Max Planck Inst Colloids & Interfaces; Milos V Novotny, Indiana Univ; Nicolle H Packer, Macquarie University; Rene Ranzinger, University of Georgia; Erdmann Rapp, Max Planck Institute of Colloids and Interfaces; Pauline M Rudd, National Institute for Bioprocessing Research and Training; Weston B Struwe, University of Oxford; Michael Tiemeyer, University of Georgia; Lance Wells, University of Georgia; William S York, University of Georgia; Joseph Zaia, Boston University; Carsten Kettner, Beilstein Institute; James C Paulson, Scripps Research Institute; Ten Feizi, Imperial College London; David Smith, Emory University
• Language: English
• Date: 2017-04-01
• Publisher: Oxford University Press (OUP)
• Publication Version: Final Published Version
• Copyright Statement: Copyright © 2016, Oxford University Press
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1093/glycob/cww118
• Title of Journal or Parent Work: Glycobiology
• ISSN: 0959-6658
• Volume: 27
• Issue: 4
• Start Page: 280
• End Page: 284
• Grant/Funding Information: Participation of R.R. and W.S.Y. is supported by the National Institute of General Medical Sciences (NIGMS) by funding the National Center for Biomedical Glycomics (NIH grant 8P41GM103490).; The participation of T.F. and Y.L. is supported by the Wellcome Trust (WT099197 and WT108430).; A.D. and S.M.H. acknowledge funding from the Biotechnology and Biological Sciences Research Council (Grant No. BB/K016164/1).; We thank the Beilstein-Institut for funding the MIRAGE initiative.; Participation of D.F.S. is supported in part by National Institutes of Health (NIH) grant R34GM116252 and the Emory Comprehensive Glycomics Core (ECGC), subsidized by the Emory University School of Medicine and by the National Center for Advancing Translational Sciences (NIH grant UL1TR000454).
• Keywords: glycan microarrays; glycomics; Life Sciences & Biomedicine; ARRAY DATA; Science & Technology; MIRAGE; EXPERIMENT MIAME; MASS-SPECTROMETRY; SPECIFICITIES; glycobiology; SHOTGUN GLYCOMICS; OLIGOSACCHARIDES; NEOGLYCOLIPIDS; BIOMARKERS; BINDING-PROTEINS; glycans; Biochemistry & Molecular Biology; RECEPTORS
• Research Categories: Chemistry, Biochemistry; Biology, Molecular

Abstract

MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26:907-910) and mass spectrometry  data (Kolarich et al. 2013, Mol. Cell Proteomics, 12:991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.