Head mounted telemetry system for seizures monitoring and sleep scoring on non-human primate

• Type of Material: Article
• Authors: JS Vuong, Emory University; JJ Garrett, Emory University; MJ Connolly, Emory University; AR York, Emory University; Robert Gross, Emory University; Annaelle Devergnas, Emory University
• Language: English
• Date: 2020-12-01
• Publisher: ELSEVIER
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2020 Elsevier B.V. All rights reserved.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.jneumeth.2020.108915
• Title of Journal or Parent Work: JOURNAL OF NEUROSCIENCE METHODS
• Volume: 346
• Start Page: 108915
• End Page: 108915
• Grant/Funding Information: Supported by a grant from the NIH Office of Research Infrastructure Programs OD P51-OD011132 to the Yerkes National Primate Research Center and a NIH/NINDS grant 1UG3NS100559-01.
• Keywords: DOG; Sleep; Life Sciences & Biomedicine; FOCAL MOTOR SEIZURES; RATS; Seizures; Neurosciences & Neurology; Telemetry; Biochemistry & Molecular Biology; Non-human primate; Science & Technology; Biochemical Research Methods; Neurosciences; SAFETY PHARMACOLOGY; EEG

Abstract

Radio telemetry systems are a useful way to continuously monitor broad electrical neuronal activity in behaving animals. It can also be used to study sleep disturbances or monitor seizure activity. Many different telemeter styles are available, but the more versatile and cost-efficient ones are the head mounted systems. They permit long-term recordings and allow more flexibility in the recordings. However, there are currently no such system available for non-human primate (NHP). In fact, the choices for NHP telemetry solutions are very limited. Here, we present a chronically implantable 3D printed chamber specifically designed to accommodate a rodent head-mounted system (RodentPACK) onto a NHP's head. We recorded EEG signal for more than a year, confirmed quality of the signal, and the ability to use the data to monitor sleep activity. We also used two of our epileptic animals to validate the embedded alarm system for real time seizure monitoring. While initially not designed for NHP, but with a minimum number of adaptions, this telemeter is in fact perfectly suitable for NHP experiments. Since early medical intervention during seizures is critical to avoid status epilepticus and to save the animal's life, real time seizures monitoring is becoming a safety requirement in many NHP studies. This method refines the current seizure monitoring methods for NHP and creates a flexible telemetry solution.