Publication

Flipping the switch: tools for detecting small molecule inhibitors of staphylococcal virulence

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Last modified
  • 02/25/2025
Type of Material
Authors
    Cassandra Quave, Emory UniversityAlexander R. Horswill, University of Iowa
Language
  • English
Date
  • 2014-12-12
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2014 Quave and Horswill.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1664-302X
Volume
  • 5
Issue
  • DEC
Start Page
  • 706
End Page
  • 706
Grant/Funding Information
  • This work was supported by a grant from the National Institutes of Health, National Center for Complementary and Alternative Medicine (R01 AT007052, PI: CLQ and Co-I: ARH).
Abstract
  • Through the expression of the accessory gene regulator quorum sensing cascade, Staphylococcus aureus is able to produce an extensive array of enzymes, hemolysins and immunomodulators essential to its ability to spread through the host tissues and cause disease. Many have argued for the discovery and development of quorum sensing inhibitors (QSIs) to augment existing antibiotics as adjuvant therapies. Here, we discuss the state-of-the-art tools that can be used to conduct screens for the identification of such QSIs. Examples include fluorescent reporters, MS-detection of autoinducing peptide production, agar plate methods for detection of hemolysins and lipase, High performance liquid chromatography-detection of hemolysins from supernatants, and cell-toxicity assays for detecting damage (or relief thereof) against human keratinocyte cells. In addition to providing a description of these various approaches, we also discuss their amenability to low-, medium-, and high-throughput screening efforts for the identification of novel QSIs.
Author Notes
  • Correspondence: Cassandra L. Quave, Department of Dermatology, Emory University School of Medicine, 1518 Clifton Road NE, CNR Building, Room 5035, Atlanta, GA 30322, USA e-mail: cquave@emory.edu.
Keywords
Research Categories
  • Biology, Microbiology
  • Health Sciences, General

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