Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

• Type of Material: Article
• Authors: Alexandria B Boehm, Stanford University; Marlene Wolfe, Emory University; Krista R Wigginton, University of Michigan; Amanda Bidwell, Stanford University; Bradley J White, Verily Life Sciences; Bridgette Hughes, Verily Life Sciences; Dorothea Duong, Verily Life Sciences; Vikram Chan-Herur, Verily Life Sciences; Heather N Bischel, University of California Davis; Colleen C Naughton, University of California Merced
• Language: English
• Date: 2023-06-22
• Publisher: NATURE PORTFOLIO
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2023
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41597-023-02297-7
• Title of Journal or Parent Work: SCIENTIFIC DATA
• Volume: 10
• Issue: 1
• Start Page: 396
• End Page: 396
• Keywords: COVID-19; SETTLED SOLIDS; Science & Technology - Other Topics; PCR; Multidisciplinary Sciences; Science & Technology; SARS-COV-2 RNA
• Research Categories: Engineering, Environmental; Engineering, Civil

Abstract

We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids in wastewater solids at twelve wastewater treatment plants in Central California, USA. Measurements were made daily for up to two years, depending on the wastewater treatment plant. Measurements were made using digital droplet (reverse-transcription–) polymerase chain reaction (RT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.