Crystal Structure of the Neisseria gonorrhoeae MtrD Inner Membrane Multidrug Efflux Pump

• Type of Material: Article
• Authors: Jani Reddy Bolla, Iowa State University; Chih-Chia Su, Iowa State University; Sylvia V. Do, Iowa State University; Abhijith Radhakrishnan, Iowa State University; Nitin Kumar, Iowa State University; Feng Long, Iowa State University; Tsung-Han Chou, Iowa State University; Jared A. Delmar, Iowa State University; Hsiang-Ting Lei Lei, Iowa State University; Kanagalaghatta Rajashankar, Cornell University; William Shafer, Emory University; Edward W. Yu, Iowa State University
• Language: English
• Date: 2014-06-05
• Publisher: Public Library of Science
• Publication Version: Final Published Version
• Copyright Statement: © 2014 Bolla et al.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0097903
• Title of Journal or Parent Work: PLoS ONE
• ISSN: 1932-6203
• Volume: 9
• Issue: 6
• Start Page: e97903
• End Page: e97903
• Grant/Funding Information: This work was supported by National Institutes of Health Grants R37AI021150 (W.M.S.) and R01GM086431 (E.W.Y.) and a Veterans Affairs Merit Award (W.M.S.) from the Medical Research Service of the Department of Veterans Affairs; Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357.; The Northeastern Collaborative Access Team beamlines of the Advanced Photon Source is supported by the National Institutes of General Medical Sciences under an award GM103403.
• Keywords: TRANSPORTER ACRB; CHANNEL; FUSION PROTEIN; Science & Technology; ESCHERICHIA-COLI; SYSTEM; MULTIDISCIPLINARY SCIENCES; BINDING POCKET; Multidisciplinary Sciences; PSEUDOMONAS-AERUGINOSA; RESISTANCE; SOFTWARE; Science & Technology - Other Topics; MECHANISM
• Research Categories: Biology, Molecular; Biology, Microbiology

Abstract

Neisseria gonorrhoeae is an obligate human pathogen and the causative agent of the sexually-transmitted disease gonorrhea. The control of this disease has been compromised by the increasing proportion of infections due to antibioticresistant strains, which are growing at an alarming rate. The MtrCDE tripartite multidrug efflux pump, belonging to the hydrophobic and amphiphilic efflux resistance-nodulation-cell division (HAE-RND) family, spans both the inner and outer membranes of N. gonorrhoeae and confers resistance to a variety of antibiotics and toxic compounds. We here report the crystal structure of the inner membrane MtrD multidrug efflux pump, which reveals a novel structural feature that is not found in other RND efflux pumps.