Interrogating the repertoire: broadening the scope of peptide-MHC multimer analysis

• Type of Material: Article
• Authors: Mark M. Davis, Stanford University School of Medicine; John D Altman, Emory University; Evan W. Newell, Stanford University School of Medicine
• Language: English
• Date: 2011-07-15
• Publisher: Nature Publishing Group
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2011 Macmillan Publishers Limited. All rights reserved
• Final Published Version (URL): http://dx.doi.org/10.1038%2Fnri3020
• Title of Journal or Parent Work: Nature Reviews Immunology
• ISSN: 1474-1733
• Volume: 11
• Issue: 8
• Start Page: 551
• End Page: 558
• Grant/Funding Information: E.W.N. is supported by a Steven and Edward Bielfelt Postdoctoral Fellowship from the American Cancer Society.; We would like to thank our sources of funding for this work. M.M.D. is supported by the Howard Hughes Medical Institute, the Bill and Melinda Gates Foundation (51731) and grants from the US National Institutes of Health (NIH; U19AI057229). J.D.A. is supported by NIH grants to the NIH Tetramer Core Facility (N01A125456M0D13) and the Emory Center for AIDS research (P30 AI050409).
• Research Categories: Health Sciences, Immunology; Biology, Microbiology

Abstract

Labelling antigen-specific T cells with peptide–MHC multimers has provided an invaluable way to monitor T cell-mediated immune responses. A number of recent developments in this technology have made these multimers much easier to make and use in large numbers. Furthermore, enrichment techniques have provided a greatly increased sensitivity that allows the analysis of the naive T cell repertoire directly. Thus, we can expect a flood of new information to emerge in the coming years.