Genetic characterization of goutanap virus, a novel virus related to negeviruses, cileviruses and higreviruses

• Type of Material: Article
• Authors: Rene Kallies, University of Bonn; Anne Kopp, University of Bonn; Florian Zirkel, University of Bonn; Alejandro Estrada, Institute of Biology of UNAM; Thomas Gillespie, Emory University; Christian Drosten, University of Bonn; Sandra Junglen, University of Bonn
• Language: English
• Date: 2014-11-12
• Publisher: MDPI
• Publication Version: Final Published Version
• Copyright Statement: © 2014 by the authors; licensee MDPI, Basel, Switzerland.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.3390/v6114346
• Title of Journal or Parent Work: Viruses
• ISSN: 1999-4915
• Volume: 6
• Issue: 11
• Start Page: 4346
• End Page: 4357
• Grant/Funding Information: This work was financially supported by the Deutsche Forschungsgemeinschaft (grant agreement number JU 2857/3-1 to Sandra Junglen).
• Keywords: Phylogeny; Cluster Analysis; Sequence Analysis, DNA; Cytopathogenic Effect, Viral; Cell Line; Culicidae; Cote d'Ivoire; Animals; Molecular Sequence Data; Mexico; High-Throughput Nucleotide Sequencing; Sequence Homology; RNA Viruses
• Research Categories: Health Sciences, Public Health; Biology, Virology

Abstract

Pools of mosquitoes collected in Côte d’Ivoire and Mexico were tested for cytopathic effects on the mosquito cell line C6/36. Seven pools induced strong cytopathic effects after one to five days post infection and were further investigated by deep sequencing. The genomes of six virus isolates from Côte d’Ivoire showed pairwise nucleotide identities of ∼99% among each other and of 56%-60% to Dezidougou virus and Wallerfield virus, two insect-specific viruses belonging to the proposed new taxon Negevirus. The novel virus was tentatively named Goutanap virus. The isolate derived from the Mexican mosquitoes showed 95% pairwise identity to Piura virus and was suggested to be a strain of Piura virus, named C6.7-MX-2008. Phylogenetic inferences based on a concatenated alignment of the methyltransferase, helicase, and RNA-dependent RNA polymerase domains showed that the new taxon Negevirus formed two monophyletic clades, named Nelorpivirus and Sandewavirus after the viruses grouping in these clades. Branch lengths separating these clades were equivalent to those of the related genera Cilevirus, Higrevirus and Blunervirus, as well as to those within the family Virgaviridae. Genetic distances and phylogenetic analyses suggest that Nelorpivirus and Sandewavirus might form taxonomic groups on genus level that may define alone or together with Cilevirus, Higrevirus and Blunervirus a viral family.