Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression

• Type of Material: Article
• Authors: Giulia Casari, University-Hospital of Modena and Reggio Emilia; Elisa Resca, Technopole Mario Veronesi; Andrea Giorgini, University-Hospital of Modena and Reggio Emilia; Olivia Candini, Rigenerand srl; Tiziana Petrachi, Technopole Mario Veronesi; Maria Serena Piccinno, Technopole Mario Veronesi; Elisabetta Manuela Foppiani, Emory University; Lucrezia Pacchioni, University-Hospital of Modena and Reggio Emilia; Marta Starnoni, University-Hospital of Modena and Reggio Emilia; Massimo Pinelli, University-Hospital of Modena and Reggio Emilia; Giorgio De Santis, University-Hospital of Modena and Reggio Emilia; Filippo Selleri, University-Hospital of Modena and Reggio Emilia; Fabio Catani, University-Hospital of Modena and Reggio Emilia; Massimo Dominici, University-Hospital of Modena and Reggio Emilia; Elena Veronesi, University-Hospital of Modena and Reggio Emilia
• Language: English
• Date: 2021-08-28
• Publisher: BMC
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2021
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1186/s13287-021-02540-1
• Title of Journal or Parent Work: Stem Cell Research & Therapy
• Volume: 12
• Grant/Funding Information: The study has been supported by Lipogems International Spa.
• Keywords: Adipose tissue; Lipogems; MSC; Microfragmentation; Osteoarthritis

Abstract

Adipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Thus, we compared AT processed by centrifugation (C-AT) to microfragmentation (MF-AT). Focusing on MF-AT, we subsequently assessed the impact of synovial fluid (SF) alone on both MF-AT and isolated AT-MSC to better understand their cartilage repair mechanisms.