Publication

Measurement of Ocular Compliance Using iPerfusion

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  • 05/14/2025
Type of Material
Authors
    Joseph M Sherwood, Imperial College LondonElizabeth M Boazak, Georgia Institute of TechnologyAndrew J Feola, Georgia Institute of TechnologyKim Parker, Imperial College LondonChristopher Ethier, Emory UniversityDarryl R Overby, Imperial College London
Language
  • English
Date
  • 2019-10-25
Publisher
  • Frontiers Media SA
Publication Version
Copyright Statement
  • © 2019 Sherwood, Boazak, Feola, Parker, Ethier and Overby.
License
Final Published Version (URL)
Title of Journal or Parent Work
Volume
  • 7
Issue
  • OCT
Start Page
  • 276
End Page
  • 276
Grant/Funding Information
  • National Institutes of Health - R21EY026685
  • RR&D Service Career Development Award - RX002342
  • National Institutes of Health - EY022359
  • National Institutes of Health - EY019696
  • Bright Focus Foundation - G2015145
  • Royal Academy of Engineering Research Fellowship, UK
Supplemental Material (URL)
Abstract
  • The pressure-volume relationship of the eye is determined by the biomechanical properties of the corneoscleral shell and is classically characterised by Friedenwald’s coefficient of ocular rigidity or, alternatively, by the ocular compliance (OC), defined as dV/dP. OC is important in any situation where the volume (V) or pressure (P) of the eye is perturbed, as occurs during several physiological and pathological processes. However, accurately measuring OC is challenging, particularly in rodents. We measured OC in 24 untreated enucleated eyes from 12 C57BL/6 mice using the iPerfusion system to apply controlled pressure steps, whilst measuring the time-varying flow rate into the eye. Pressure and flow data were analysed by a “Discrete Volume” (integrating the flow trace) and “Step Response” method (fitting an analytical solution to the pressure trace). OC evaluated at 13 mmHg was similar between the two methods (Step Response, 41 [37, 46] vs. Discrete Volume, 42 [37, 48] nl/mmHg; mean [95% CI]), although the Step Response Method yielded tighter confidence bounds on individual eyes. OC was tightly correlated between contralateral eyes (R2 = 0.75, p = 0.0003). Following treatment with the cross-linking agent genipin, OC decreased by 40 [33, 47]% (p = 0.0001; N = 6, Step Response Method). Measuring OC provides a powerful tool to assess corneoscleral biomechanics in mice and other species.
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Research Categories
  • Engineering, Biomedical
  • Health Sciences, Medicine and Surgery

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