Maintenance of CD4+ T cell TCR Vβ repertoire heterogeneity is characteristic of apathogenic SIV infection in non-human primate model of AIDS

• Type of Material: Article
• Authors: Pavel Bostik, Emory University; Susan T Stephenson, Emory University; Rebecca M Lynch, Emory University; Adriana Cardona, Emory University; Aftab A Ansari, Emory University
• Language: English
• Date: 2007-12-20
• Publisher: Elsevier
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2007 Elsevier Inc. All rights reserved.
• License: Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1016/j.virol.2007.07.019
• Title of Journal or Parent Work: Virology
• ISSN: 0042-6822
• Volume: 369
• Issue: 2
• Start Page: 324
• End Page: 328
• Grant/Funding Information: This work was supported by the NIH RO1 AI65362 to P.B.
• Keywords: SIV; V beta; AIDS; CD4+ T cell; T cell receptor
• Research Categories: Health Sciences, Epidemiology; Biology, Cell; Health Sciences, Pathology

Abstract

A PCR based assay was established to screen for potential changes in the heterogeneity of Vβ TCR expressing CD4+ T cells from rhesus macaques (RM) and sooty mangabeys (SM) prior to and following SIV infection. The rationale for these studies was to determine whether the progressive CD4+ T cell loss in SIV infected disease susceptible RM and the moderate CD4+ T cell loss in disease resistant SM leads to the depletion of select Vβ TCR families of CD4+ T cells. Results show that whereas SIV infection leads to the loss of Vβ TCR heterogeneity in disease susceptible RM, the CD4+ T cells from SM retain their degree of Vβ TCR heterogeneity, suggesting that the mechanism(s) of SIV induced CD4+ T cell loss maybe distinct in these 2 species and contribute to the differences in the clinical outcome.