Plasma CXCL8 and MCP-1 as surrogate plasma biomarkers of latent tuberculosis infection among household contacts–A cross-sectional study

• Type of Material: Article
• Authors: Sivaprakasam T. Selvavinayagam, State Public Health Laboratory, Chennai; Bijulal Aswathy, Central University of Tamil Nadu; Yean K. Yong, Xiamen University Malaysia; Asha Frederick, National Tuberculosis Elimination Programme, Chennai; Lakshmi Murali, National Tuberculosis Elimination Programme, Chennai; Vasudevan Kalaivani, State Public Health Laboratory, Chennai; Sree J. Karishma, Central University of Tamil Nadu; Manivannan Rajeshkumar, State Public Health Laboratory, Chennai; Adukkadukkam Anusree, Central University of Tamil Nadu; Meganathan Kannan, Central University of Tamil Nadu; Natarajan Gopalan, Central University of Tamil Nadu; Ramachandran Vignesh, Universiti Kuala Lumpur; Amudhan Murugesan, The Government Theni Medical College and Hospital; Hong Yien Tan, Xiamen University Malaysia; Ying Zhang, Xiamen University Malaysia; Samudi Chandramathi, University of Malaya; Munusamy Ponnan Sivasankaran, Seattle Children's Research Institute; Pachamuthu Balakrishnan, Saveetha Institute of Medical and Technical Sciences; Sakthivel Govindaraj, Emory University; Siddappa N. Byrareddy, University of Nebraska, Omaha; Vijayakumar Velu, Emory University; Marie Larsson, Linkoping University; Esaki M. Shankar, central University of Tamil Nadu; Sivadoss Raju, State Public Health Laboratory, Chennai
• Language: English
• Date: 2023-11-22
• Publisher: PLOS
• Publication Version: Final Published Version
• Copyright Statement: © 2023 Selvavinayagam et al
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): https://doi.org/10.1371/journal.pgph.0002327
• Title of Journal or Parent Work: PLOS Global Public Health
• Volume: 3
• Issue: 11
• Start Page: e0002327
• Grant/Funding Information: S. T. S. and S. R. are funded by the National Health Mission, Tamil Nadu (680/NGS/NHMTNMSC/ENGG/2021) for the Directorate of Public Health and Preventive Medicine. E.M.S. is funded by the Department of Science and Technology-Science and Engineering Research Board, Government of India (CRG/2019/006096). This work is also supported by grants through AI52731, the Swedish Research Council, the Swedish, Physicians against AIDS Research Foundation, the Swedish International Development Cooperation Agency, SIDA SARC, VINNMER for Vinnova, Linköping University Hospital Research Fund, CALF, and the Swedish Society of Medicine (to ML). H. Y. T. is supported by Xiamen University Research Funding (XMUMRF/2020-C5/ITCM/0003 and VV is supported by: The NIH Office of Research Infrastructure Programs (P51 OD011132 to ENPRC), and Emory CFAR (P30 AI050409).
• Keywords: plasma cytokines; household contacts; tuberculosis infection
• Research Categories: Health Sciences, Immunology; Biology, Microbiology; Health Sciences, Public Health

Abstract

Early detection of latent tuberculosis infection (LTBI) is critical to TB elimination in the current WHO vision of End Tuberculosis Strategy. The study investigates whether detecting plasma cytokines could aid in diagnosing LTBI across household contacts (HHCs) positive for IGRA, HHCs negative for IGRA, and healthy controls. The plasma cytokines were measured using a commercial Bio-Plex Pro Human Cytokine 17-plex assay. Increased plasma CXCL8 and decreased MCP-1, TNF-α, and IFN-γ were associated with LTBI. Regression analysis showed that a combination of CXCL8 and MCP-1 increased the risk of LTBI among HHCs to 14-fold. Our study suggests that CXCL-8 and MCP-1 could serve as the surrogate biomarkers of LTBI, particularly in resource-limited settings. Further laboratory investigations are warranted before extrapolating CXCL8 and MCP-1 for their usefulness as surrogate biomarkers of LTBI in resource-limited settings.