Profiling of Glycan Receptors for Minute Virus of Mice in Permissive Cell Lines Towards Understanding the Mechanism of Cell Recognition

• Type of Material: Article
• Authors: Sujata Halder, University of Florida; Susan Cotmore, Yale University; Jamie Heimburg-Molinaro, Emory University; David Smith, Emory University; Richard Cummings, Emory University; Xi Chen, University of California Davis; Alana J. Trollope, Imperial College London; Simon J. North, Imperial College London; Stuart M. Haslam, Imperial College London; Anne Dell, Imperial College London; Peter Tattersall, Yale University; Robert McKenna, University of Florida; Mavis Agbandje-McKenna, University of Florida
• Language: English
• Date: 2014-01-27
• Publisher: Public Library of Science
• Publication Version: Final Published Version
• Copyright Statement: © 2014 Halder et al.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0086909
• Title of Journal or Parent Work: PLoS ONE
• ISSN: 1932-6203
• Volume: 9
• Issue: 1
• Start Page: e86909
• End Page: e86909
• Grant/Funding Information: This work was supported by National Science Foundation [MCB-0718948 to R.M. and M.A-M.], University of Florida College of Medicine Research Funds to [M.A-M.], National Institute of Health [CA029303 to S.C. and P.T., GM085448 to D.F.S., and GM076360 to X.C.].
• Keywords: TISSUE TROPISM; STRAIN; Science & Technology; BOVINE PARVOVIRUS; SIALIC-ACID BINDING; TRANSDUCTION; Multidisciplinary Sciences; MOUSE-BRAIN; DEPENDENT EXPRESSION; CANCER METASTASIS; MULTIDISCIPLINARY SCIENCES; Science & Technology - Other Topics; ENTRY; GENE-TRANSFER
• Research Categories: Biology, Molecular; Chemistry, Biochemistry

Abstract

The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 Galβ1-4GlcNAc motif (Neu5Acα2-3Galβ1-4GlcNAc or 3′SIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3′SIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3′SIA-Le X identified in a previous glycan microarray screen.