Publication

Neonatal Colonic Inflammation Increases Spinal Transmission and Cystathionine beta-Synthetase Expression in Spinal Dorsal Horn of Rats with Visceral Hypersensitivity

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Last modified
  • 03/05/2025
Type of Material
Authors
    Liting Zhao, Soochow UniversityYing Xiao, Soochow UniversityRui-Xia Weng, Soochow UniversityXuelian Liu, Soochow UniversityPing-An Zhang, Soochow UniversityChuang-Ying Hu, Soochow UniversityShan Yu, Emory UniversityGuang-Yin Xu, Soochow University
Language
  • English
Date
  • 2017-10-04
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2017 Zhao, Xiao, Weng, Liu, Zhang, Hu, Yu and Xu.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1663-9812
Volume
  • 8
Start Page
  • 696
End Page
  • 696
Grant/Funding Information
  • This work was supported by grants from National Natural Science Foundation of China (81230024 and 81471137 to GY-X, 31300909 to YX).
Abstract
  • Irritable bowel syndrome (IBS) is a common gastrointestinal disorder characterized by chronic abdominal pain and alteration of bowel movements. The pathogenesis of visceral hypersensitivity in IBS patients remains largely unknown. Hydrogen sulfide (H 2 S) is reported to play an important role in development of visceral hyperalgesia. However, the role of H 2 S at spinal dorsal horn level remains elusive in visceral hypersensitivity. The aim of this study is designed to investigate how H 2 S takes part in visceral hypersensitivity of adult rats with neonatal colonic inflammation (NCI). Visceral hypersensitivity was induced by neonatal colonic injection of diluted acetic acid. Expression of an endogenous H2S synthesizing enzyme cystathionine ß-synthetase (CBS) was determined by Western blot. Excitability and synaptic transmission of neurons in the substantia gelatinosa (SG) of spinal cord was recorded by patch clamping. Here, we showed that expression of CBS in the spinal dorsal horn was significantly upregulated in NCI rats. The frequency of glutamatergic synaptic activities in SG was markedly enhanced in NCI rats when compared with control rats. Application of NaHS increased the frequency of both spontaneous and miniature excitatory post-synaptic currents of SG neurons in control rats through a presynaptic mechanism. In contrast, application of AOAA, an inhibitor of CBS, dramatically suppressed the frequency of glutamatergic synaptic activities of SG neurons of NCI rats. Importantly, intrathecal injection of AOAA remarkably attenuated visceral hypersensitivity of NCI rats. These results suggest that H2S modulates pain signaling likely through a presynaptic mechanism in SG of spinal dorsal horn, thus providing a potential therapeutic strategy for treatment for chronic visceral pain in patients with IBS.
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Keywords
Research Categories
  • Health Sciences, Pharmacy
  • Biology, Neuroscience
  • Health Sciences, Pharmacology

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