Examination of Galectin Localization Using Confocal Microscopy

• Type of Material: Article
• Authors: Daniel Giuliano Cerri, University of Sao Paulo; Connie Arthur, Emory University; Lilian Cataldi Rodrigues, University of Sao Paulo; Marise Lopes Fermino, University of Sao Paulo; Lenaldo Branco Rocha, Triangulo Mineiro Federal University; Sean Stowell, Emory University; Marcelo Dias-Baruffi, University of Sao Paulo
• Language: English
• Date: 2015-01-01
• Publisher: Springer Nature
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © Springer Science+Business Media New York 2015.
• Final Published Version (URL): http://dx.doi.org/10.1007/978-1-4939-1396-1_23
• Title of Journal or Parent Work: Galectins: Methods in Molecular Biology
• Volume: 1207
• Start Page: 343
• End Page: 354
• Grant/Funding Information: None declared
• Keywords: Biochemical Research Methods; Science & Technology; Leukocytes; Biochemistry & Molecular Biology; Immunofluorescence; Sensitivity; Confocal microscopy; Expression; Galectin; Tissue; Life Sciences & Biomedicine; Antibody
• Research Categories: Chemistry, Biochemistry; Biology, Microbiology; Health Sciences, Immunology

Abstract

Confocal microscopy provides a unique modality to examine the expression and localization of biomolecules in a variety of settings. Using this technique, an image is acquired from the focal plane of the objective using focused laser light, making it possible to work within the resolution limit of the optical system. In addition, by acquiring multiple images from a variety of focal planes, stacked series of images can provide clear spatial localization of a probed structure or protein. We describe herein the immunofl uorescence methods for galectin staining in frozen sections of tissue for galectin localization using confocal microscopy.