Transcriptomic dissection of tongue squamous cell carcinoma

• Type of Material: Article
• Authors: Hui Ye, University of Illinois at Chicago; Tianwei Yu, Emory University; Stephane Temam, University of Texas
• Language: English
• Date: 2008
• Publisher: BioMed Central
• Publication Version: Final Published Version
• Copyright Statement: © 2008 Ye et al; licensee BioMed Central Ltd.
• License: Creative Commons Attribution 2.0 Generic
• Final Published Version (URL): http://www.biomedcentral.com/1471-2164/9/69
• Title of Journal or Parent Work: BMC Genomics
• ISSN: 1471-2164
• Volume: 9
• Issue: 69
• Grant/Funding Information: This work was supported in part by NIH PHS grants K22 DE014847, RO3 DE016569, and RO3 CA114688 (to X. Zhou), R01 DE015970 (to D. Wong), P01 CA106451 Project 3 (to L. Mao), and a Foundation de France grant (to S. Temam). This work was supported in part by NIH PHS grants K22 DE014847, RO3 DE016569, and RO3 CA114688 (to X. Zhou), R01 DE015970 (to D. Wong), P01 CA106451 Project 3 (to L. Mao), and a Foundation de France grant (to S. Temam).
• Research Categories: Biology, Biostatistics; Health Sciences, Oncology; Health Sciences, Public Health

Abstract

Background The head and neck/oral squamous cell carcinoma (HNOSCC) is a diverse group of cancers, which develop from many different anatomic sites and are associated with different risk factors and genetic characteristics. The oral tongue squamous cell carcinoma (OTSCC) is one of the most common types of HNOSCC. It is significantly more aggressive than other forms of HNOSCC, in terms of local invasion and spread. In this study, we aim to identify specific transcriptomic signatures that associated with OTSCC. Results Genome-wide transcriptomic profiles were obtained for 53 primary OTSCCs and 22 matching normal tissues. Genes that exhibit statistically significant differences in expression between OTSCCs and normal were identified. These include up-regulated genes (MMP1, MMP10, MMP3, MMP12, PTHLH, INHBA, LAMC2, IL8, KRT17, COL1A2, IFI6, ISG15, PLAU, GREM1, MMP9, IFI44, CXCL1), and down-regulated genes (KRT4, MAL, CRNN, SCEL, CRISP3, SPINK5, CLCA4, ADH1B, P11, TGM3, RHCG, PPP1R3C, CEACAM7, HPGD, CFD, ABCA8, CLU, CYP3A5). The expressional difference of IL8 and MMP9 were further validated by real-time quantitative RT-PCR and immunohistochemistry. The Gene Ontology analysis suggested a number of altered biological processes in OTSCCs, including enhancements in phosphate transport, collagen catabolism, I-kappaB kinase/NF-kappaB signaling cascade, extracellular matrix organization and biogenesis, chemotaxis, as well as suppressions of superoxide release, hydrogen peroxide metabolism, cellular response to hydrogen peroxide, keratinization, and keratinocyte differentiation in OTSCCs. Conclusion In summary, our study provided a transcriptomic signature for OTSCC that may lead to a diagnosis or screen tool and provide the foundation for further functional validation of these specific candidate genes for OTSCC.