Spatial control of robust transgene expression in mouse artery endothelium under ultrasound guidance

• Type of Material: Article
• Authors: Renfa Liu, Peking University; Shuai Qu, Peking University; Yunxue Xu, Peking University; Hanjoong Jo, Emory University; Zhifei Dai, Peking University
• Language: English
• Date: 2022-07-18
• Publisher: SPRINGERNATURE
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2022
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41392-022-01031-w
• Title of Journal or Parent Work: SIGNAL TRANSDUCTION AND TARGETED THERAPY
• Volume: 7
• Issue: 1
• Start Page: 225
• End Page: 225
• Keywords: Biochemistry & Molecular Biology; Science & Technology; Cell Biology; Life Sciences & Biomedicine
• Research Categories: Engineering, Biomedical

Abstract

Dear Editor, Dysfunctional vascular endothelial cells (ECs) contribute to the pathophysiology of several cardiovascular diseases, such as atherosclerosis and its life-threatening complications.1 Gene therapy can be a valuable approach to modulate endothelial cell function for the prevention of atherosclerosis. However, there is still a lack of method for transgene expression in vascular endothelium.2 Herein, an effective and specific strategy was established for noninvasive spatial control of transgene expression into the target region of the mouse artery without systemic spillover using an ultrasound and microbubble (MB) guided adeno-associated viral vector (UMGAAV) (Supplementary Fig. S1). To the best of our knowledge, this is the first report on noninvasive spatial control of transgene expression in arterial endothelium in vivo.