Transcriptome Analysis of Acetyl-Homoserine Lactone-Based Quorum Sensing Regulation in Yersinia pestis

• Type of Material: Article
• Authors: Christopher LaRock, Emory University; Jing Yu, Iowa State University; Alexander R. Horswill, University of Iowa; Matthew R. Parsek, University of Washington; F. Chris Minion, Iowa State University
• Language: English
• Date: 2013-04-19
• Publisher: Public Library of Science
• Publication Version: Final Published Version
• Copyright Statement: © 2013 LaRock et al.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0062337
• Title of Journal or Parent Work: PLoS ONE
• ISSN: 1932-6203
• Volume: 8
• Issue: 4
• Start Page: e62337
• End Page: e62337
• Grant/Funding Information: This work was supported by the Office of Naval Research Grant N00014-06-1-1176 to FCM, ARH, and MRP.
• Keywords: ISOCITRATE LYASE ACTIVITY; PSEUDOTUBERCULOSIS; PASTEURELLA-PESTIS; PLAGUE; Science & Technology; Multidisciplinary Sciences; INFECTION; BORNE TRANSMISSION; HEAT-SHOCK; FLEA VECTOR; GENE-EXPRESSION; Science & Technology - Other Topics; PERSISTENCE
• Research Categories: Biology, Microbiology; Biology, Genetics

Abstract

The etiologic agent of bubonic plague, Yersinia pestis, senses self-produced, secreted chemical signals in a process named quorum sensing. Though the closely related enteric pathogen Y. pseudotuberculosis uses quorum sensing system to regulate motility, the role of quorum sensing in Y. pestis has been unclear. In this study we performed transcriptional profiling experiments to identify Y. pestis quorum sensing regulated functions. Our analysis revealed that acyl-homoserine lactone-based quorum sensing controls the expression of several metabolic functions. Maltose fermentation and the glyoxylate bypass are induced by acyl-homoserine lactone signaling. This effect was observed at 30°C, indicating a potential role for quorum sensing regulation of metabolism at temperatures below the normal mammalian temperature. It is proposed that utilization of alternative carbon sources may enhance growth and/or survival during prolonged periods in natural habitats with limited nutrient sources, contributing to maintenance of plague in nature.