Microfluidic Amperometric Sensor for Analysis of Nitric Oxide in Whole Blood

• Type of Material: Article
• Authors: Rebecca A. Hunter, University of North Carolina; Benjamin J. Privett, University of North Carolina; W. Hampton Henley, University of North Carolina; Elise R. Breed, Emory University; Zhe Liang, Emory University; Rohit Mittal, Emory University; Benyam P. Yoseph, Emory University; Jonathan E. McDunn, University of North Carolina; Eileen Burd, Emory University; Craig Coopersmith, Emory University; J. Michael Ramsey, University of North Carolina; Mark H. Schoenfisch, University of North Carolina
• Language: English
• Date: 2013-06-18
• Publisher: American Chemical Society
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2013 American Chemical Society.
• Final Published Version (URL): http://dx.doi.org/10.1021/ac400932s
• Title of Journal or Parent Work: Analytical Chemistry
• ISSN: 0003-2700
• Volume: 85
• Issue: 12
• Start Page: 6066
• End Page: 6072
• Grant/Funding Information: The authors acknowledge research support from the National Institutes of Health (NIH NIAID A13-0212, GM072808 (CMC), and GM095442 (RM and BPY)).
• Keywords: ANGIOGENESIS; Physical Sciences; ELECTROCHEMICAL DETECTION; CHEMICAL BIOLOGY; Chemistry, Analytical; SEPSIS; PATHOPHYSIOLOGY; FLUORESCENCE; REAL-TIME; Chemistry; Science & Technology; RELEASE; MICROSENSOR; PSEUDOMONAS-AERUGINOSA PNEUMONIA
• Research Categories: Chemistry, Analytical; Health Sciences, Medicine and Surgery

Abstract

Standard photolithographic techniques and a nitric oxide (NO) selective xerogel polymer were utilized to fabricate an amperometric NO microfluidic sensor with low background noise and the ability to analyze NO levels in small sample volumes (∼250 μL). The sensor exhibited excellent analytical performance in phosphate buffered saline, including a NO sensitivity of 1.4 pA nM-1, a limit of detection (LOD) of 840 pM, and selectivity over nitrite, ascorbic acid, acetaminophen, uric acid, hydrogen sulfide, ammonium, ammonia, and both protonated and deprotonated peroxynitrite (selectivity coefficients of -5.3, -4.2, -4.0, -5.0, -6.0, -5.8, -3.8, -1.5, and -4.0, respectively). To demonstrate the utility of the microfluidic NO sensor for biomedical analysis, the device was used to monitor changes in blood NO levels during the onset of sepsis in a murine pneumonia model.