PlexinA4 Distribution in the Adult Rat Spinal Cord and Dorsal Root Ganglia

• Type of Material: Article
• Authors: Claire-Anne Gutekunst, Emory University; Eric N. Stewart, Emory University; Colin K. Franz, University of Calgary; Arthur W English, Emory University; Robert E. Gross, Emory University
• Language: English
• Date: 2012-05
• Publisher: Elsevier
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2012 Elsevier B.V. All rights reserved.
• License: Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1016/j.jchemneu.2012.03.002
• Title of Journal or Parent Work: Journal of Chemical Neuroanatomy
• ISSN: 0891-0618
• Volume: 44
• Issue: 1
• Start Page: 1
• End Page: 13
• Grant/Funding Information: This work was supported by a grant from NIH (K08 NS46322-01A1) to REG. CAG is funded in part by NIH (R03 NS58376-01A1).
• Keywords: Semaphorin; Rattus norvegicus; plexin-a4; plxna4; spinal cord injury; plexa4; protein distribution; axon guidance molecule
• Research Categories: Biology, Neuroscience

Abstract

PlexinsA1–A4 participate in class 3 semaphorin signaling as co-receptors to neuropilin 1 and 2, PlexinA4 being the latest member of the PlexinA subfamily to be identified. Little is known about the cellular distribution of PlexinA4 in the spinal cord and dorsal root ganglion (DRG). Here, immunohistochemical studies using antibodies to PlexinA4 revealed immunolabeling in neurons in both dorsal and, to a greater extent, ventral horns of the spinal cord. Ventral horn PlexinA4 positive neurons exhibited morphology, size, and location consistent with both motor neurons and interneurons. Labeling was found in motor axons exiting through the ventral roots, and more widespread labeling was observed in ascending and descending white matter tracts. Within the DRG, immunostaining was observed in neuronal cell bodies as well as the central and peripheral processes of these cells. PlexinA4 is expressed in the peripheral nervous system where its expression is regulated upon nerve injury. This is the first detailed description of the cellular and subcellular distribution of PlexinA4 in the adult spinal cord and DRG, and it will set the basis for future studies on the potential role of PlexinA4 in regeneration and repair of the adult central and peripheral nervous system.