Multi-institutional study of nuclear KIFC1 as a biomarker of poor prognosis in African American women with triple-negative breast cancer

• Type of Material: Article
• Authors: Angela Ogden, Georgia State University; Chakravarthy Garlapati, Georgia State University; Xiaoxian Li, Emory University; Ravi Chakra Turaga, Georgia State University; Gabriela Oprea, Emory University; Nikita Wright, Georgia State University; Shristi Bhattarai, Georgia State University; Karuna Mittal, Georgia State University; Ceyda Sönmez Wetherilt, Georgia State University; Uma Krishnamurti, Emory University; Michelle Reid, Emory University; Mildred Jones, Northside Hosp Canc Inst; Meenakshi Gupta, West Georgia Med Ctr; Remus Osan, Georgia State University; Sonal Pattni, Emory University; Ansa Riaz, Georgia State University; Sergey Klimov, Georgia State University; Arundhati Rao, BSWHealth; Guilherme Cantuaria, Northside Hosp Canc Inst; Padmashree C. G. Rida, Georgia State University; Ritu Aneja, Georgia State University
• Language: English
• Date: 2017-02-20
• Publisher: Nature Research (part of Springer Nature): Fully open access journals
• Publication Version: Final Published Version
• Copyright Statement: © 2017 The Author(s).
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/srep42289
• Title of Journal or Parent Work: Scientific Reports
• ISSN: 2045-2322
• Volume: 7
• Issue: 1
• Start Page: 42289
• End Page: 42289
• Grant/Funding Information: This work was supported by grants to RA by from the NCI (R01 CA169127) and NIMHD (R41 MD010303).
• Keywords: PROTEIN; KINESIN; VESICLE TRANSPORT; CELLS; Multidisciplinary Sciences; EXTRA CENTROSOMES; HSET; SPINDLE; MICROTUBULES; MECHANISMS; ETHNICITY; Science & Technology - Other Topics; Science & Technology
• Research Categories: Biology, General; Health Sciences, Pathology

Abstract

Nuclear KIFC1 (nKIFC1) predicts worse outcomes in breast cancer, but its prognostic value within racially distinct triple-negative breast cancer (TNBC) patients is unknown. Thus, nKIFC1 expression was assessed by immunohistochemistry in 163 African American (AA) and 144 White TNBC tissue microarrays (TMAs) pooled from four hospitals. nKIFC1 correlated significantly with Ki67 in White TNBCs but not in AA TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. High nKIFC1 weighted index (WI) was associated with significantly worse overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS) (Hazard Ratios [HRs] = 3.5, 3.1, and 3.8, respectively; P = 0.01, 0.009, and 0.007, respectively) in multivariable Cox models in AA TNBCs but not White TNBCs. Furthermore, KIFC1 knockdown more severely impaired migration in AA TNBC cells than White TNBC cells. Collectively, these data suggest that nKIFC1 WI an independent biomarker of poor prognosis in AA TNBC patients, potentially due to the necessity of KIFC1 for migration in AA TNBC cells.