Toward quantification of hypoxia using fluorinated EuII/III-containing ratiometric probes

• Type of Material: Article
• Authors: S A Amali S Subasinghe, Wayne State University; Caitlyn J Ortiz, Baylor College of Medicine; Jonathan Romero, Baylor College of Medicine; Cassandra L Ward, Wayne State University; Alexander G Sertage, Wayne State University; Lyazat Kurenbekova, Baylor College of Medicine; Jason Yustein, Emory University; Robia G Pautler, Baylor College of Medicine; Matthew J Allen, Wayne State University
• Language: English
• Date: 2023-04-05
• Publisher: Proceedings of the National Academy of Sciences
• Publication Version: Final Published Version
• Copyright Statement: © 2023 the Author(s). Published by PNAS.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): https://doi.org/10.1073/pnas.2220891120
• Title of Journal or Parent Work: Proceedings of the National Academy of Sciences
• Volume: 120
• Issue: 15
• Start Page: e2220891120
• Grant/Funding Information: We acknowledge the NIH (R01EB026453) for financial support. The Lumigen Instrument Center X-ray crystallography lab is partially supported by the NIH (3R01EB027103-02S1), and NMR lab is partially supported by the NIH (S10OD028488). A.G.S. was supported by the NIH (T32GM142519).
• Keywords: responsive contrast agents; ratiometric measurements; MRI; hypoxia; ratiometric analysis
• Research Categories: Health Sciences, Oncology; Health Sciences, Radiology

Abstract

This study reports dual-mode probes that enable ratiometric quantification of hypoxia in a concentration-independent manner based on the magnetic and electronic differences between EuII and EuIII. These results are significant because hypoxia is an important biomarker in a wide range of diseases and there is a critical need to spatially quantify hypoxia to improve our biological understanding of these diseases and identify and evaluate new treatments.