Publication

Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter

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Last modified
  • 02/25/2025
Type of Material
Authors
    Nealen G. Laxpati, Emory UniversityBabak Mahmoudi, Emory UniversityClaire-Anne Gutekunst, Emory UniversityJonathan P. Newman, Emory UniversityRiley Zeller-Townson, Emory UniversityRobert Gross, Emory University
Language
  • English
Date
  • 2014-10-29
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2014 Laxpati, Mahmoudi, Gutekunst, Newman, Zeller-Townsonand and Gross.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1662-6443
Volume
  • 7
Issue
  • October
Start Page
  • 40
End Page
  • 40
Grant/Funding Information
  • This work was funded by a seed grant from the Emory Neurosciences Initiative, support from the American Epilepsy Society, Translational Neurology research fellowships to Nealen G. Laxpati and Babak Mahmoudi (5T32NS7480-12), Epilepsy Research Foundation predoctoral fellowship to Nealen G. Laxpati, NSF GRFP Fellowship 08-593 and NSF IGERT Fellowship DGE-0333411 to Jonathan P. Newman. Riley Zeller-Townson was supported by NSF EFRI #1238097, NIH 1R01NS079757-01, and the ASEE SMART Fellowship.
Abstract
  • Optogenetic channels have greatly expanded neuroscience's experimental capabilities, enabling precise genetic targeting and manipulation of neuron subpopulations in awake and behaving animals. However, many barriers to entry remain for this technology - including low-cost and effective hardware for combined optical stimulation and electrophysiologic recording. To address this, we adapted the open-source NeuroRighter multichannel electrophysiology platform for use in awake and behaving rodents in both open and closed- loop stimulation experiments. Here, we present these cost-effective adaptations, including commercially available LED light sources; custom-made optical ferrules; 3D printed ferrule hardware and software to calibrate and standardize output intensity; and modifications to commercially available electrode arrays enabling stimulation proximally and distally to the recording target. We then demonstrate the capabilities and versatility of these adaptations in several open and closed-loop experiments, demonstrate spectrographic methods of analyzing the results, as well as discuss artifacts.
Author Notes
  • Correspondence: Robert E. Gross, Department of Neurosurgery, Emory University School of Medicine, 1365 Clifton Road, NE Suite 6200, Atlanta, GA 30322, USA, e-mail: rgross@emory.edu.
Keywords
Research Categories
  • Biology, Neuroscience
  • Engineering, Biomedical

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