Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli

• Type of Material: Article
• Authors: Alexandra dos Anjos Cassado, Universidade Federal de São Paulo; José Antônio Tavares de Albuquerque, Universidade Federal de São Paulo; Luiz Roberto Sardinha, Instituto Israelita de Ensino e Pesquisa Albert Einstein; Carina de Lima Buzzo, Universidade Federal de São Paulo; Lucas Faustino, Universidade Federal de São Paulo; Rogério Nascimento, Universidade Federal de São Paulo; Eliver Ghosn, Emory University; Maria Regina D'Imperio Lima, Universidade Federal de São Paulo; Jose Maria Mosig Alvarez, Universidade Federal de São Paulo; Karina Ramalho Bortoluci, Universidade Federal de São Paulo
• Language: English
• Date: 2011-07-22
• Publisher: Public Library of Science
• Publication Version: Final Published Version
• Copyright Statement: © 2011 Cassado et al.
• License: Creative Commons Attribution 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0022141
• Title of Journal or Parent Work: PLoS ONE
• ISSN: 1932-6203
• Volume: 6
• Issue: 7
• Start Page: e22141
• End Page: e22141
• Keywords: IN-VIVO; HETEROGENEITY; FLOW-CYTOMETRY; PATTERN-RECOGNITION RECEPTORS; ALTERNATIVELY ACTIVATED MACROPHAGES; Multidisciplinary Sciences; IFN-GAMMA; Science & Technology - Other Topics; MECHANISMS; ANTIGEN PRESENTATION; Science & Technology; POPULATIONS; DENDRITIC CELLS
• Research Categories: Biology, Genetics; Health Sciences, Immunology

Abstract

The peritoneal cavity (PerC) is a singular compartment where many cell populations reside and interact. Despite the widely adopted experimental approach of intraperitoneal (i.p.) inoculation, little is known about the behavior of the different cell populations within the PerC. To evaluate the dynamics of peritoneal macrophage (MØ) subsets, namely small peritoneal MØ (SPM) and large peritoneal MØ (LPM), in response to infectious stimuli, C57BL/6 mice were injected i.p. with zymosan or Trypanosoma cruzi. These conditions resulted in the marked modification of the PerC myelo-monocytic compartment characterized by the disappearance of LPM and the accumulation of SPM and monocytes. In parallel, adherent cells isolated from stimulated PerC displayed reduced staining for β-galactosidase, a biomarker for senescence. Further, the adherent cells showed increased nitric oxide (NO) and higher frequency of IL-12-producing cells in response to subsequent LPS and IFN-γ stimulation. Among myelo-monocytic cells, SPM rather than LPM or monocytes, appear to be the central effectors of the activated PerC; they display higher phagocytic activity and are the main source of IL-12. Thus, our data provide a first demonstration of the consequences of the dynamics between peritoneal MØ subpopulations by showing that substitution of LPM by a robust SPM and monocytes in response to infectious stimuli greatly improves PerC effector activity.