Combined Use of Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses Is a Powerful Diagnostic Tool of Active Tuberculosis

• Type of Material: Article
• Authors: Virginie Rozot, Lausanne University Hospital; Amelio Patrizia, Lausanne University Hospital; Selena Vigano, Lausanne University Hospital; Jesica Mazza-Stalder, Lausanne University Hospital; Elita Idrizi, Lausanne University Hospital; Cheryl Liane Day, Emory University; Matthieu Perreau, Lausanne University Hospital; Catherine Lazor-Blanchet, Lausanne University Hospital; Khalid Ohmiti, Lausanne University Hospital; Delia Goletti, National Institute for Infectious Diseases, Italy; Pierre-Alexandre Bart, Lausanne University Hospital; Willem Hanekom, University of Cape Town; Thomas J. Scriba, University of Cape Town; Laurent Nicod, Lausanne University Hospital; Giuseppe Pantaleo, Lausanne University Hospital; Alexandre Harari, Lausanne University Hospital
• Language: English
• Date: 2015-02-01
• Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option C
• Publication Version: Final Published Version
• Copyright Statement: © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1093/cid/ciu795
• Title of Journal or Parent Work: Clinical Infectious Diseases
• ISSN: 1058-4838
• Volume: 60
• Issue: 3
• Start Page: 432
• End Page: 437
• Grant/Funding Information: The research leading to these results has received funding from the Swiss Vaccine Research Institute and the European Community's Seventh Framework Programme (FP7/2007-2013 and FP7/2007-2011) under European Commission Grant Agreement (number 241642).
• Keywords: Microbiology; active tuberculosis disease; CHILDREN; SIGNATURE; CD8 T cells; diagnosis; Life Sciences & Biomedicine; Immunology; ASSAY; LATENT INFECTION; latent Mtb infection; HUMANS; Science & Technology; functional profile; Infectious Diseases; DISEASE
• Research Categories: Health Sciences, Epidemiology; Health Sciences, Immunology

Abstract

Immune-based assays are promising tools to help to formulate diagnosis of active tuberculosis. A multiparameter flow cytometry assay assessing T-cell responses specific to Mycobacterium tuberculosis and the combination of both CD4 and CD8 T-cell responses accurately discriminated between active tuberculosis and latent infection.