Probing Virus-Glycan Interactions Using Glycan Microarrays

• Type of Material: Article
• Authors: Jamie Heimburg-Molinaro, Emory University; Mary Tappert, University of Oklahoma; Xuezheng Song, Emory University; Yi Lasanajak, Emory University; Gillian Air, University of Oklahoma; David Smith, Emory University; Richard Cummings, Emory University
• Language: English
• Date: 2012-01-01
• Publisher: Humana Press
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2012, Springer Science+Business Media, LLC
• Final Published Version (URL): http://dx.doi.org/10.1007/978-1-61779-373-8_18
• Title of Journal or Parent Work: Carbohydrate Microarrays
• Volume: 808
• Start Page: 251
• End Page: 267
• Keywords: H3N2 INFLUENZA-VIRUSES; Glycan microarrays; ARRAY; Biochemical Research Methods; Glycan-binding motif; Glycomics; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; Science & Technology; Fluorescence detection; NEURAMINIDASE; SPECIFICITY; Influenza virus
• Research Categories: Biology, Molecular; Chemistry, Biochemistry

Abstract

Glycan microarrays are surfaces that contain immobilized oligosaccharides or glycoconjugates and have proven useful in probing the interactions between glycan-binding proteins (GBPs) and individual glycans. Such glycan microarrays have been especially important in studying virus-glycan interactions, as most viruses express one or more GBPs important for pathogenesis. For studying interactions of influenza viruses with glycans, we describe protocols for fluorescent labeling of virus, addition of virus to a glycan microarray, analysis of a glycan microarray slide experiment, and interpretation of data. © 2012 Springer Science+Business Media, LLC.