The central role of EED in the orchestration of polycomb group complexes

• Type of Material: Article
• Authors: Qi Cao, University of Michigan; Xiaoju Wang, University of Michigan; Meng Zhao, Emory University; Rendong Yang, Emory University; Rohit Malik, University of Michigan; Yuanyuan Qiao, University of Michigan; Anton Poliakov, University of Michigan; Anastasia K. Yocum, University of Michigan; Yong Li, University of Michigan; Wei Chen, University of Michigan; Xuhong Cao, University of Michigan; Xia Jiang, University of Michigan; Arun Dahiya, University of Michigan; Clair Harris, University of Michigan; Felix Y. Feng, University of Michigan; Sundeep Kalantry, University of Michigan; Zhaohui Qin, Emory University; Saravana M. Dhanasekaran, University of Michigan; Arul M. Chinnaiyan, University of Michigan
• Language: English
• Date: 2014-01-01
• Publisher: Nature Research (part of Springer Nature): Fully open access journals
• Publication Version: Final Published Version
• Copyright Statement: © 2014 Macmillan Publishers Limited. All rights reserved.
• Final Published Version (URL): http://dx.doi.org/10.1038/ncomms4127
• Title of Journal or Parent Work: Nature Communications
• ISSN: 2041-1723
• Volume: 5
• Issue: 1
• Start Page: 3127
• End Page: 3127
• Grant/Funding Information: A.M.C. is supported by the Prostate Cancer Foundation (PCF); the Doris Duke Charitable Foundation; and the Howard Hughes Medical Institute.; This work is supported in part by the Prostate SPORE P50CA69568; and National Institutes of Health (R01CA132874).; A.M.C. is an American Cancer Society Research Professor and A. Alfred Taubman Scholar. Q.C. is supported by the U.S. Department of Defense (PC094725); F.Y.F. is supported by a Young Investigator Award from the PCF; S.K. is supported by an NIH Director’s New Innovator Award (DP2-OD-008646-01) and a March of Dimes Basil O’Connor Starter Scholar Research Award; M.Z. and Z.S.Q. are supported by National Institutes of Health (7R01HG005119-02).
• Keywords: CANCER; HISTONE METHYLTRANSFERASE ACTIVITY; EZH2; TARGET GENES; METHYLATION; Science & Technology; H2A UBIQUITYLATION; Multidisciplinary Sciences; CPG ISLANDS; STATISTICAL-MODEL; Science & Technology - Other Topics; PROTEIN EED; PRC2
• Research Categories: Health Sciences, Pathology; Health Sciences, Medicine and Surgery; Biology, Biostatistics

Abstract

Polycomb repressive complexes 1 and 2 (PRC1 and 2) play a critical role in the epigenetic regulation of transcription during cellular differentiation, stem cell pluripotency and neoplastic progression. Here we show that the polycomb group protein EED, a core component of PRC2, physically interacts with and functions as part of PRC1. Components of PRC1 and PRC2 compete for EED binding. EED functions to recruit PRC1 to H3K27me3 loci and enhances PRC1-mediated H2A ubiquitin E3 ligase activity. Taken together, we suggest an integral role for EED as an epigenetic exchange factor coordinating the activities of PRC1 and 2.