RNA Virus Reverse Genetics and Vaccine Design

• Type of Material: Article
• Authors: Christopher C. Stobart, Emory University; Martin L Moore, Emory University
• Language: English
• Date: 2014-07
• Publisher: MDPI
• Publication Version: Final Published Version
• Copyright Statement: © 2014 by the authors; licensee MDPI, Basel, Switzerland.
• License: Creative Commons Attribution 3.0 Unported
• Final Published Version (URL): http://www.mdpi.com/1999-4915/6/7/2531
• Title of Journal or Parent Work: Viruses
• ISSN: 1999-4915
• Volume: 6
• Issue: 7
• Start Page: 2531
• End Page: 2550
• Grant/Funding Information: This work was supported by NIH grants R01AI087798 and U19AI095227.
• Keywords: vaccines; reverse genetics; RNA virus
• Research Categories: Health Sciences, General

Abstract

RNA viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. The development of reverse genetics systems for manipulation and study of RNA virus genomes has provided platforms for designing and optimizing viral mutants for vaccine development. Here, we review the impact of RNA virus reverse genetics systems on past and current efforts to design effective and safe viral therapeutics and vaccines.