Differential expression of chemokine receptors on monocytes in TB and HIV S

• Type of Material: Article
• Authors: Wegene Tamene, Ethiopian Public Health Institute, Addis Ababa; Vincent C Marconi, Emory University; Meseret Abebe, Armauer Hansen Research Institute, Addis Ababa; Liya Wassie, Armauer Hansen Research Institute, Addis Ababa; Yohannes Belay, Ethiopian Public Health Institute, Addis Ababa; Amha Kebede, Ethiopian Public Health Institute, Addis Ababa; Ulrich Sack, Leipzig University; Rawleigh Howe, rmauer Hansen Research Institute, Addis Ababa
• Language: English
• Date: 2023-06-01
• Publisher: CELL PRESS
• Publication Version: Final Published Version
• Copyright Statement: © 2023 The Authors
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.heliyon.2023.e17202
• Title of Journal or Parent Work: HELIYON
• Volume: 9
• Issue: 6
• Start Page: e17202
• End Page: e17202
• Grant/Funding Information: Research reported in this publication was funded by EPHI and AHRI partially through the EDCTP (IP. 09.32040.011) and National Institutes of Health (NIH)-Fogarty-Ethiopia Emory TB Research Training Program" (D43TW009127) grants. The content is solely the responsibility of the authors and does not necessarily represent the official views of any of the aforementioned institutions.
• Keywords: PULMONARY; IMMUNE ACTIVATION; Science & Technology - Other Topics; Monocyte subset; MYCOBACTERIUM-TUBERCULOSIS; TB; HETEROGENEITY; Multidisciplinary Sciences; INFECTION; DEFICIENCY; Science & Technology; CCR2; Chemokine receptor; PREFERENTIALLY HARBORS HIV-1; TRAFFICKING; HIV; CELLS
• Research Categories: Health Sciences, Medicine and Surgery; Health Sciences, Public Health; Health Sciences, Immunology

Abstract

In the present study, we defined multiple chemokine receptors expressed by classical, intermediate and non-classical monocyte subsets in TB, HIV and TB/HIV co-infection and associate it with the perturbation of monocyte subsets due to the diseases. Peripheral blood mononuclear cells from TB+ (n = 34), HIV+ (n = 35), TB + HIV+ (n = 12), as well as TB-HIV- healthy controls (n = 39), were tested for monocyte phenotyping by flow cytometry. Frequencies of intermediate and non-classical monocytes were significantly higher in TB and/or HIV disease relative to healthy controls. CCR2 and CX3CR1 were significantly higher on monocytes in TB disease, whereas CCR4 and CCR5 were present at higher levels in HIV disease. TB/HIV co-infected patients exhibited CCR2, CCR5 and CX3CR1 levels intermediate to TB and HIV subjects, while CCR4 was at a higher level than HIV. Despite the increase in the expression of chemokine receptors due to disease conditions, chemokine receptors maintained their original expression pattern on monocyte subsets. Our data provided new insight into the disease-specific but not monocyte subsets-specific modulation of chemokine receptors in TB and HIV.