Mincle and human B cell function

• Type of Material: Article
• Authors: Kazuhito Kawata, University of California Davis; Petr Illarionov, University of Birmingham; Thomas Kenny, University of California Davis; Weici Zhang, University of California Davis; Masanobu Tsuda, University of California Davis; Yugo Ando, University of California Davis; Patrick Leung, University of California Davis; Aftab A Ansari, Emory University; M. Eric Gershwin, University of California Davis
• Language: English
• Date: 2012-12-01
• Publisher: Elsevier
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2012 Elsevier Ltd.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.jaut.2012.04.004
• Title of Journal or Parent Work: Journal of Autoimmunity
• ISSN: 0896-8411
• Volume: 39
• Issue: 4
• Start Page: 315
• End Page: 322
• Grant/Funding Information: Financial support provided by National Institutes of Health grant DK39588
• Keywords: FUNGAL-INFECTION; C-TYPE LECTIN; INNATE IMMUNE-RESPONSE; Immunology; Mincle; RECOGNITION; EXPRESSION; INDUCTION; Science & Technology; C-type lectin; CANDIDA-ALBICANS; DENDRITIC CELLS; Toll-like receptor; TOLL-LIKE RECEPTORS; ACTIVATING RECEPTOR; Life Sciences & Biomedicine
• Research Categories: Health Sciences, Immunology; Biology, Cell

Abstract

C-type lectin receptors are pattern recognition receptors that are critical for autoimmunity and the immune response. Mincle is a C-type lectin receptor expressed by a variety of antigen presenting cells including macrophages, neutrophils, dendritic cells and B cells; a variety of stimuli including stress are known to induce the expression of Mincle. Mincle is an FcRγ-associated activation receptor that senses damaged cells and upon ligation induces activated macrophages to produce inflammatory cytokines. Recently, while several studies have reported that Mincle plays an important role in macrophage responses to fungal infection its function on B cells remains to be defined. In efforts to elucidate the function of Mincle expressed by B cells, we studied the expression of Mincle on subsets of B cells and analyzed cytokines and synthesized immunoglobulin upon ligation of Mincle. The expression of Mincle on CD27-CD19 + naïve B cells is significantly higher than CD27 + CD19 + memory B cells. The stimulation of TLR9 ligand induced Mincle expression on B cells. Furthermore, co-stimulation of TLR9 and Mincle ligand reduced IgG and IgA production from B cells without a significant change in the inflammatory cytokines TNF-α, IL-6, IL-8 and IL-10. Our data identifies Mincle as a potentially critical player in human B cell responses.