Environmental exposure to pyrethroids and sperm sex chromosome disomy: a cross-sectional study

• Type of Material: Article
• Authors: Heather A Young, George Washington University; John D Meeker, University of Michigan; Sheena E Martenies, George Washington University; Zaida I Figueroa, George Washington University; Dana Boyd Barr, Emory University; Melissa J Perry, George Washington University
• Language: English
• Date: 2013
• Publisher: BMC (part of Springer Nature)
• Publication Version: Final Published Version
• Copyright Statement: © 2013 Young et al.; licensee BioMed Central Ltd.
• License: Creative Commons Attribution 2.0 Generic
• Final Published Version (URL): http://www.ehjournal.net/content/12/1/111
• Title of Journal or Parent Work: Environmental Health
• ISSN: 1476-069X
• Volume: 12
• Issue: 111
• Grant/Funding Information: This work was supported by Grants ES 009718, ES 000002, and ES017457.
• Keywords: In situ hybridization; Fluorescence; Endocrine Disruptors; Pyrethroids; Uniparental Disomy; Aneuploidy
• Research Categories: Health Sciences, Public Health; Environmental Sciences

Abstract

Background The role of environmental pesticide exposures, such as pyrethroids, and their relationship to sperm abnormalities are not well understood. This study investigated whether environmental exposure to pyrethroids was associated with altered frequency of sperm sex chromosome disomy in adult men. Methods A sample of 75 subjects recruited through a Massachusetts infertility clinic provided urine and semen samples. Individual exposures were measured as urinary concentrations of three pyrethroid metabolites ((3-phenoxybenzoic acid (3PBA), cis- and trans- 3-(2,2-Dichlorovinyl)-1-methylcyclopropane-1,2-dicarboxylic acid (CDCCA and TDCCA)). Multiprobe fluorescence in situ hybridization for chromosomes X, Y, and 18 was used to determine XX, YY, XY, 1818, and total sex chromosome disomy in sperm nuclei. Poisson regression analysis was used to examine the association between aneuploidy rates and pyrethroid metabolites while adjusting for covariates. Results Between 25-56% of the sample were above the limit of detection (LOD) for the pyrethroid metabolites. All sex chromosome disomies were increased by 7-30% when comparing men with CDCCA and TDCCA levels above the LOD to those below the LOD. For 3PBA, compared to those below the LOD, those above the LOD had YY18 disomy rates 1.28 times higher (95% CI: 1.15, 1.42) whereas a reduced rate was seen for XY18 and total disomy (IRR = 0.82; 95% CI: 0.77, 0.87; IRR = 0.93; 95% CI: 0.87-0.97), and no association was seen for XX18 and 1818. Conclusions Our findings suggest that urinary concentrations of CDCCA and TDCCA above the LOD were associated with increased rates of aneuploidy. However the findings for 3BPA were not consistent. This is the first study to examine these relationships, and replication of our findings is needed before the association between pyrethroid metabolites and aneuploidy can be fully defined. Keywords: Aneuploidy, Uniparental Disomy, Endocrine Disruptors, In situ hybridization, Fluorescence, Pyrethroids