Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand

• Type of Material: Article
• Authors: Mohan Maddur, Emory University; Meenu Sharma, Université de Technologie de Compiègne; Pushpa Hegde, Université de Technologie de Compiègne; Emmanuel Stephen-Victor, Institut National de la Santé et de la Recherche Médicale Unité; Bali Pulendran, Emory University; Srini V. Kaveri, Institut National de la Santé et de la Recherche Médicale Unité; Jagadeesh Bayry, Institut National de la Santé et de la Recherche Médicale Unité
• Language: English
• Date: 2014-06-01
• Publisher: Nature Publishing Group
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2014 Macmillan Publishers Limited. All rights reserved
• Final Published Version (URL): http://dx.doi.org/10.1038/ncomms5092
• Title of Journal or Parent Work: Nature Communications
• ISSN: 2041-1723
• Volume: 5
• Start Page: 4092
• End Page: 4092
• Grant/Funding Information: This study was supported by the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement HEALTH-2010.2.4.5-2 ALLFUN
• Keywords: INTRAVENOUS IMMUNOGLOBULIN; MULTIDISCIPLINARY SCIENCES; CD4(+) T-CELLS; Multidisciplinary Sciences; LYMPHOCYTES; DIFFERENTIATION; Science & Technology - Other Topics; OX40 LIGAND; ACTIVATION; IN-VIVO; EXPRESSION; Science & Technology; RESPONSES; ADAPTIVE IMMUNITY
• Research Categories: Health Sciences, General; Health Sciences, Immunology

Abstract

Dendritic cells (DCs) play a critical role in immune homeostasis by regulating the functions of various immune cells, including T and B cells. Notably, DCs also undergo education on reciprocal signalling by these immune cells and environmental factors. Various reports demonstrated that B cells have profound regulatory functions, although only few reports have explored the regulation of human DCs by B cells. Here we demonstrate that activated but not resting B cells induce maturation of DCs with distinct features to polarize Th2 cells that secrete interleukin (IL)-5, IL-4 and IL-13. B-cell-induced maturation of DCs is contact dependent and implicates signalling of B-cell activation molecules CD69, B-cell-activating factor receptor, and transmembrane activator and calcium-modulating cyclophilin ligand interactor. Mechanistically, differentiation of Th2 cells by B-cell-matured DCs is dependent on OX-40 ligand. Collectively, our results suggest that B cells have the ability to control their own effector functions by enhancing the ability of human DCs to mediate Th2 differentiation.