Utility of a Viral Vesicular Panel Multiplex Polymerase Chain Reaction Assay for the Diagnosis of Monkeypox, Herpes Simplex, and Varicella Zoster Viruses.

• Type of Material: Article
• Authors: Eli Wilber, Emory University; Paulina Rebolledo, Emory University; Vyjayanti Kasinathan, Emory University; Stephanie Merritt, Grady Memorial Hospital; Boghuma Titanji, Emory University; Bruce Aldred, Emory University; Sheetal Kandiah, Emory University; Susan M. Ray, Emory University; Anandi Sheth, Emory University; Jonathan A. Colasanti, Emory University; Yun F. Wang, Emory University
• Language: English
• Date: 2023-03
• Publisher: Oxford University Press
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1093/ofid/ofad140
• Title of Journal or Parent Work: Open Forum Infect Dis
• Volume: 10
• Issue: 3
• Start Page: ofad140
• End Page: ofad140
• Grant/Funding Information: A. N. S. and B. K. T. are supported by the Center for AIDS Research at Emory University (grant number P30AI050409). B. K. T. is supported by the National Institutes of Health (Building Interdisciplinary Research Careers in Women’s Health grant).
• Keywords: vesicular panel; mpox diagnosis; mpox; multiplex PCR assay
• Research Categories: Health Sciences, Public Health; Biology, Virology

Abstract

Mpox (monkeypox) represents a diagnostic challenge due to varied clinical presentations and multiple mimics. A commercially available multiplex polymerase chain reaction panel accurately detects mpox virus as well as common mimics (herpes simplex virus, varicella zoster virus) in clinical specimens and could be used in routine clinical, surveillance, and outbreak settings.